phospho-ALK (Tyr1278 + Tyr1282 + Tyr1283)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3021R
英文名称phospho-ALK (Tyr1278 + Tyr1282 + Tyr1283)
中文名称磷酸化间变型**瘤激酶抗体
别 名ALK (Phospho-Tyr1278/1282/1283); ALK (phospho-Y1278/1282/1283); p-ALK(Tyr1278/1282/1283); p-ALK(Y1278/1282/1283); ALK tyrosine kinase receptor precursor; ALK/EML4 fusion gene, included; ALK/NPM1 fusion gene, included; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki 1; Anaplastic lymphoma kinase Ki1; Anaplastic lymphoma kinase p80 CD 246; CD246; CD246 antigen; EC 2.7.10.1 Ki 1; Ki1; NBLST3; Tcrz; TFG/ALK; ALK_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 染色质和核信号 神经生物学 信号转导 细胞凋亡 生长因子和** 激酶和磷酸酶 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
phospho-ALK (Tyr1278 + Tyr1282 + Tyr1283)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量174kDa
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ALK around the phosphorylation site of Tyr1278/1282/1283:DI(p-Y)RAS(p-Y)(p-Y)RK
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ALK (Tyr1278 + Tyr1282 + Tyr1283)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), LK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011].
Function:
Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
Subunit:
Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1.
phospho-ALK (Tyr1278 + Tyr1282 + Tyr1283)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ALK (Tyr1278 + Tyr1282 + Tyr1283)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Runx3 Runx3抗体
合格 MBP 髓鞘碱性蛋白/磷脂碱性蛋白抗体
合格 合格 GLUT4 葡萄糖转运蛋白4抗体
合格 合格 ARC 即刻早期基因ARC抗体
合格 IFN gamma γ-干扰素/γ-IFN抗体
合格 CD133 造血干细胞抗原CD133抗体
合格 VCAM-1 血管内皮细胞粘附分子抗体
合格 合格 EGFR 表皮生长因子受体抗体
合格 合格 MMP2 基质金属蛋白酶2抗体
合格 合格 MMP-3 基质金属蛋白酶3抗体
合格 TIMP-1 金属蛋白酶组织抑制因子-1抗体
合格 TIMP-4 金属蛋白酶组织抑制因子-4抗体
合格 Tau 微管相关蛋白抗体
合格 DR3 死亡受体3抗体
合格 MMP1 基质金属蛋白酶-1抗体
合格 合格 Myelin-oligodendrocyte glycoprotein 髓鞘少树突胶质细胞糖蛋白抗体
合格 DISC1 (NT) DISC1 N端抗体
合格 WNK4 WNK4抗体
合格 DISC1 (CT) DISC1 C端抗体
合格 OPG 骨保护蛋白/护骨素抗体
合格 合格 ACE 血管紧张素转换酶ACE1抗体
合格 合格 ChRM2 毒蕈碱型乙酰胆碱受体M2抗体
合格 ACTH (1-39) 促肾上腺皮质**(1-39)抗体
合格 IL1RAPL1 白介素1受体相关蛋白样1前体蛋白抗体
合格 合格 合格 Annexin V 重组膜粘连蛋白5抗体
合格 HSL/LIPE ***敏感脂肪酶抗体
合格 合格 phospho-SMAD2(Ser425) 磷酸化细胞信号转导分子SMAD2抗体
合格 Thioredoxin 硫氧还蛋白抗体
合格 合格 Cytochrome P450 2J3 细胞色素P450 2J3抗体
合格 MMP1 基质金属蛋白酶-1抗体
合格 合格 合格 NFKB p65 细胞核因子/k基因结合核因子抗体
合格 Ghrelin 脑肠肽抗体
合格 CD31 血小板内皮细胞黏附分子-1抗体
合格 Adiponectin 脂联素抗体
合格 合格 合格 GLUT1 葡萄糖转运蛋白1抗体
合格 VSIG4 T**细胞负调节蛋白抗体
合格 IFN gamma 干扰素-γ/IFN-γ抗体