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产品编号bs-8203R
英文名称Nestin
中文名称巢蛋白/神经上皮干细胞蛋白抗体
别 名ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.
规格100ul 200ul
研究领域细胞生物 发育生物学 神经生物学 干细胞 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Cow,
alpha-Synuclein抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
alpha-Synuclein抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
alpha-Synuclein抗体溶解方法：
1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：
溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；
若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。
溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）
的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。
3、抗体的分装
抗体溶解后，将其分装成 5-10支（以0.1mlalpha-Synuclein抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。
另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
alpha-Synuclein抗体关联产品列表：
bs-0016R YES 合格 合格 Tyrosine Hydroxylase 酪氨酸羟化酶抗体
bs-0017R No 合格 Galanin 甘丙肽/神经节肽抗体
bs-0018R No 合格 IDE 胰岛素降解酶抗体
bs-0019R YES 合格 合格 osteopontin 骨桥蛋白/分泌型磷蛋白1抗体
bs-0022R YES 合格 合格 ERK1 + ERK2 丝裂原活化蛋白激酶1/ERK 1/2抗体
bs-0023M No 合格 GSK-3 beta 糖原合酶激酶-3β抗体
bs-0024R No 合格 合格 presenilin 1 早老素蛋白-1抗体
bs-0026R YES 合格 osteopontin 骨桥蛋白/分泌型磷蛋白1抗体
bs-0027R YES 合格 Melatonin Receptor 1A 褪黑素受体1A/松果体素受体1A抗体
bs-0028R YES 合格 GSK-3 Beta 糖原合酶激酶-3β抗体
bs-0029R YES 合格 PP2A alpha + beta 蛋白质磷酸酶-2A抗体
bs-0031R No 合格 合格 BTG2 B细胞迁移基因2抗体
bs-0032R YES 合格 合格 合格 Bcl-2 Bcl-2抗体