phospho-Amyloid Precursor Protein (Thr668)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3028R
英文名称phospho-Amyloid Precursor Protein (Thr668)
中文名称磷酸化APP淀粉样肽前体蛋白抗体
别 名Amyloid Precursor Protein (phospho T668); Amyloid Precursor Protein (phospho Thr668); p-Amyloid Precursor Protein (phospho T668); APP (Phospho-Thr668); p-APP(Thr668); C31; C80; C83; C99; beta-amyloid precursor protein C-terminus; A4 amyloid protein; A4; AAA; ABETA; ABPP; AD 1; AD1; Alzheimer disease 1; Alzheimer disease; Alzheimer's disease amyloid protein; Amyloid beta (A4) precursor protein; Amyloid beta A4 protein; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta A4 protein precursor isoform a; Amyloid beta A4 protein precursor isoform b; Amyloid beta A4 protein precursor isoform c; Amyloid beta protein; Amyloid beta-peptide; Amyloid of aging and alzheimer disease; APP; APP I ; APPI; Beta amyloid peptide; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Human mRNA for amyloid A4 precursor of Alzheimer's disease; PN 2; PN II; PN2; PreA4; Protease nexin II; A4_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域细胞生物 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶 Alzheimer's
抗体来源Rabbit
克隆类型Polyclonal
phospho-Amyloid Precursor Protein (Thr668)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量3.4-9/83kDa
细胞定位细胞核 细胞浆 细胞膜 细胞外基质
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human APP around the phosphorylation site of Thr668:AV(p-T)PE <Extracellular>
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Amyloid Precursor Protein (Thr668)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Function:
Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons.
Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity.
Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brai.
The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).
phospho-Amyloid Precursor Protein (Thr668)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Amyloid Precursor Protein (Thr668)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Geminin DNA复制抑制因子抗体
合格 C6orf174 6号染色体开放阅读框174抗体
合格 CCZ1 CCZ1蛋白抗体
合格 合格 PGGT1A 蛋白香叶烯基转移酶1α抗体
合格 Cerebral protein 5 脑蛋白5抗体
合格 合格 GTPBP9 鸟嘌呤核苷酸结合蛋白9抗体
合格 合格 KRCC1 含赖氨酸卷曲螺旋蛋白1抗体
合格 VMAT2 脑单胺类神经递质转运蛋白抗体
合格 Copine-6 神经网蛋白CopineVI抗体
合格 Rheumatoid Factor 类风湿因子抗体
合格 STAT1 p84+p91 信号转导与转录激活因子1抗体
合格 Alpha-synuclein (nitro-Tyr39) 硝基化α-突触核蛋白/n-syn抗体
合格 PDE4B 磷酸二酯酶4B抗体
合格 PCTP PCTP蛋白抗体
合格 PINX-1 端粒酶抑制因子PinX-1抗体
合格 合格 Cdc25C 细胞分裂周期蛋白25C抗体
合格 RBM20 RNA结合蛋白20抗体
合格 ZNF828 锌指蛋白828抗体
合格 PIBF1 孕酮诱导阻断因子1抗体
合格 C14ORF140 14号染色体开放阅读框140抗体
合格 C14orf130 14号染色体开放阅读框130抗体
合格 C15orf62 15号染色体开放阅读框62抗体
合格 合格 PARP16 多腺苷二磷酸多聚酶16抗体
合格 合格 RPUSD2 RNA尿苷酸合酶结构域蛋白2抗体
合格 KLHL36 Kelch样蛋白36抗体
合格 C16orf48 16号染色体开放阅读框48抗体
合格 PDZD9 PDZ结构域PDZK9蛋白抗体
合格 SLC39A11 溶质载体家族39成员11抗体
合格 XTP4 乙型肝炎病毒X蛋白反转录蛋白4抗体
合格 C17orf42 17号染色体开放阅读框42抗体
合格 合格 C17orf57 17号染色体开放阅读框57抗体
合格 C17orf74 17号染色体开放阅读框74抗体
合格 C17orf75 17号染色体开放阅读框75抗体
合格 C17orf77 17号染色体开放阅读框77抗体
合格 C17orf78 17号染色体开放阅读框78抗体
合格 C17orf82 17号染色体开放阅读框82抗体
合格 合格 C17orf97 17号染色体开放阅读框97抗体
合格 合格 pan 14-3-3 14-3-3蛋白抗体
合格 RIMKLA 核糖体蛋白S6修饰样蛋白A抗体
合格 FAM76B FAM76B蛋白抗体
合格 ENAH ENAH蛋白抗体
合格 EGFL8 表皮生长因子样蛋白8抗体