phospho-ATF2 (Ser322)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5169R
英文名称phospho-ATF2 (Ser322)
中文名称磷酸化活化复制因子2抗体
别 名ATF2 (phospho Ser322); ATF2 (phospho S322); p-ATF2 (S322); p-ATF2 (Ser322); CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_MOUSE.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 染色质和核信号 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-ATF2 (Ser322)抗体交叉反应 Human, Mouse, Rat, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser322:NT(p-S)GR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ATF2 (Ser322)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
Function:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Subunit:
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
Subcellular Location:
Nucleus.
Tissue Specificity:
Abundant expression seen in the brain.
Post-translational modifications:
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4.
Similarity:
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
SWISS:
P16951
Gene ID:
100047997
phospho-ATF2 (Ser322)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ATF2 (Ser322)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 LRRC62 富含亮氨酸重复蛋白62抗体
合格 合格 MFAP1 微原纤维胶原相关蛋白1抗体
合格 NEDD1 神经前体细胞表达蛋白1抗体
合格 NEK8 丝氨酸/苏氨酸蛋白激酶NEK8抗体
合格 NSP5/NSP 5 alpha 3 alpha 核结构蛋白5抗体
合格 PELO PELO蛋白抗体
合格 合格 SUGT1 SUGT1蛋白抗体
合格 phospho-APC6 (Ser560) 磷酸化细胞分裂周期蛋白16抗体
合格 ZWINT 着丝粒ZW10相互作用蛋白1抗体
合格 FAM3C 上皮分泌型FAM3C蛋白抗体
合格 SPAG8 精子相关抗原8抗体
合格 SUPT16H 染色体特异性转录延伸因子抗体
合格 Cyclin K 周期素K抗体
合格 Cyclin J 周期素J抗体
合格 CABLES1 细胞周期蛋白依赖性激酶CABLES1抗体
合格 CDCA3 细胞分裂周期相关蛋白3抗体
合格 CDC2L5 细胞分裂周期2样蛋白激酶5抗体
合格 合格 phospho-FRS2(Tyr436) 磷酸化成纤维细胞生长因子受体底物2抗体
合格 CLK2 细胞分裂周期样激酶2抗体
合格 DUSP26 双特异性蛋白磷酸酶26/丝裂原活化蛋白激酶磷酸酶8抗体
合格 phospho-LATS1+LATS2 (Thr1079 +Thr1041) 磷酸化肿瘤抑制基因LATS1/LATS2抗体
合格 ANKRD28 锚蛋白重复域28抗体
合格 SIPA1L2 信号诱导增殖相关蛋白1样蛋白2抗体
合格 PPP2R1A 蛋白质磷酸酶2调节亚基1A抗体
合格 NIFK NIFK蛋白抗体
合格 Ubiquitin 泛素蛋白抗体
合格 ANKRD11 锚蛋白重复结构域蛋白11抗体
合格 BTBD7 BTB/POZ结构域蛋白7抗体
合格 CCDC102B 卷曲螺旋结构域蛋白102B抗体
合格 CCDC80 卷曲螺旋结构域蛋白80抗体
合格 Zona pellucida glycoprotein 3 卵母细胞透明带糖蛋白3抗体
合格 合格 ACSBG2 长链脂肪酸辅酶A连接酶ACSBG2抗体
合格 WNT 5B 信号通路Wnt-5a抗体
合格 ZNF570 锌指蛋白570抗体
合格 合格 ADCK5 ADCK5蛋白抗体
合格 CLN3 神经细胞蜡样质脂褐质沉积病蛋白CLN3抗体
合格 CKLFSF7 趋化素样因子超家族成员7抗体
合格 CNKSR1 Ras激酶抑制因子连接增强蛋白1抗体
合格 HIAT1 海马丰富基因转录蛋白1抗体
合格 KLHL24 Kelch样蛋白24抗体
合格 合格 KLHL3 Kelch样蛋白3抗体