phospho-ATF2 (Ser62 / Ser44)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5170R
英文名称phospho-ATF2 (Ser62 / Ser44)
中文名称磷酸化活化复制因子2抗体
别 名ATF2 (phospho S62 + S44); ATF2(Phospho-Ser44); ATF2 (phospho Ser44); ATF2 (phospho S44); p-ATF2 (S44); p-ATF2 (Ser44); CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 染色质和核信号 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-ATF2 (Ser62 / Ser44)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser44 / Ser62 (human):ND(p-S)VI
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ATF2 (Ser62 / Ser44)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
Function:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Subunit:
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
Subcellular Location:
Nucleus.
Tissue Specificity:
Abundant expression seen in the brain.
Post-translational modifications:
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4.
Similarity:
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
SWISS:
P15336
Gene ID:
1386
phospho-ATF2 (Ser62 / Ser44)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ATF2 (Ser62 / Ser44)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 MFF 线粒体裂变因子MFF抗体
合格 MTCH1 细胞增殖诱导蛋白60（早老素相关蛋白）
合格 MTFR1 线粒体裂变调节蛋白1抗体
合格 FUT1 岩藻糖转移酶1抗体
合格 ICAM4 细胞间粘附分子4抗体
合格 XKR1 膜转运蛋白XK抗体
合格 HCA57 肝癌相关抗原57抗体
合格 DFFB DNA断裂因子抗体（蛋白酶激活脱氧核糖核酸酶）
合格 DNase II 脱氧核糖核酸酶2抗体
合格 FLI1 尤文肉瘤FLI1蛋白抗体
合格 NUP50 核孔蛋白50抗体
合格 SUZ12 胚胎干细胞抑制蛋白Suz12抗体
合格 合格 DR6 肿瘤细胞调亡素/肿瘤坏死因子受体死亡受体6抗体
合格 Corin 心脏特异性丝氨酸蛋白酶抗体
合格 GPR55 蛋白偶联受体55抗体
合格 P2RX4 三磷酸腺苷门控阳离子通道蛋白抗体
合格 Renalase 肾胺酶抗体
合格 AXUD1 转化生长因子β诱导蛋白3抗体（半胱氨酸和丝氨酸富含核蛋白1）
合格 BS69 腺病毒5结合蛋白BS69/骨形态发生蛋白受体相关分子1抗体
合格 ZBTB2 锌指蛋白437抗体
合格 CENPP 着丝粒蛋白P抗体
合格 Importin4 核蛋白4抗体（Ran结合蛋白4）
合格 CDCA1 细胞分裂周期相关蛋白1抗体
合格 CAPG2 染色体相关蛋白2抗体
合格 REC8 减数分裂重组蛋白家族REC8抗体
合格 CAPD3 浓缩素2复合亚基D3抗体
合格 Anillin 胞环蛋白肌动蛋白结合蛋白抗体
合格 DCTN3 动力蛋白激活蛋白亚基3抗体
合格 NAT10 乙酰基转移酶10抗体
合格 SPECC1L 细胞质和纺锤体机化蛋白A抗体
合格 VPS4a 液泡分选蛋白4抗体
合格 CDC123 细胞分裂周期蛋白CDC123抗体
合格 CDC20B 细胞分裂周期蛋白20B抗体
合格 CKS2 细胞周期蛋白依赖性激酶调节亚基2抗体
合格 DSN1 着丝粒相关蛋白DSN1抗体