phospho-ATM (Ser794)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 12545R
英文名称phospho-ATM (Ser794)
中文名称磷酸化****扩张性共济失调症突变蛋白抗体
别 名ATM (Phospho-Ser794); ATM (phospho S794); p-ATM (Ser794); p-ATM (S794); AT complementation group A; AT complementation group C; AT complementation group D; AT complementation group E; AT mutated; AT protein;AT1;ATA;Ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; ATC; ATDC; ATE; ATM; Human phosphatidylinositol 3 kinase homolog; Serine protein kinase ATM; T cell prolymphocytic leukemia; TEL1; TPLL; ATM_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-ATM (Ser794)抗体交叉反应 Human,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量350kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human ATM around the phosphorylation site of Ser794:KK(p-S)PN
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ATM (Ser794)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
Function:
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.
Subunit:
Dimers or tetramers in inactive state. On DNA damage, autophosphorylation dissociates ATM into monomers rendering them catalytically active. Binds p53/TP53, ABL1, BRCA1, NBN/nibrin and TERF1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with RAD17; DNA damage promotes the association. Interacts with EEF1E1; the interaction, induced on DNA damage, up-regulates TP53. Interacts with DCLRE1C, KAT8, KAT5, NABP2, ATMIN and CEP164. Interacts with AP2B1 and AP3B2; the interaction occurs in cytoplasmic vesicles (By similarity). Interacts with TELO2 and TTI1. Interacts with DDX1.
phospho-ATM (Ser794)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ATM (Ser794)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 VGF 神经生长因子诱导蛋白抗体
合格 BRF1 RNA聚合酶III抗体
合格 Glutamine PRPP amidotransferase 谷氨酰胺磷酸核糖基焦磷酸酰胺基转移酶
合格 DDIT4L DNA损伤诱导转录样蛋白4抗体
合格 Gas1 生长休止特定蛋白1抗体
合格 STK18 丝氨酸/苏氨酸蛋白激酶18
合格 SCGB3A1 结合珠蛋白家族3A1抗体
合格 BMP1 骨形态发生蛋白1/胶原C蛋白肽链内切酶抗体
合格 MRC2 巨噬细胞甘露糖受体2抗体
合格 phospho-PI3 Kinase p110 beta(Ser1070) 磷酸化磷脂酰肌醇激酶（PI3Kβ）抗体
合格 phospho-PP2A alpha+beta(Tyr307) 磷酸化蛋白磷酸酶2A（PP2Aα）抗体
合格 ICBP90/UHRF1 核蛋白95抗体
合格 PPP4C 丝氨酸/苏氨酸蛋白磷酸酶4C抗体
合格 ZnT-1 锌转运蛋白1抗体
合格 NPD014 1号染色体开放阅读框63抗体
合格 CRABP1/Retinol binding protein 视黄醇结合蛋白抗体
合格 合格 SOX10 转录因子SOX10抗体
合格 合格 Phospho-Paxillin(Ser83) 磷酸化桩蛋白Paxillin抗体
合格 合格 caspase-8 subunit p18 半胱氨酸蛋白酶8抗体
合格 合格 kir 6.1 ATP敏感钾离子通道蛋白抗体
合格 Phospholipase C beta 2 磷酯酶Cβ2抗体
合格 合格 合格 CD95/FAS 载脂蛋白1抗体
合格 CH25H 胆固醇25羟化酶抗体
合格 Testin 肿瘤抑制基因Testin抗体
合格 PAK2 p21激活激酶2抗体
合格 CENPH 着丝粒蛋白H抗体
合格 CKAP4 细胞骨架相关蛋白4抗体
合格 CKMT 酸性线粒体肌酸激酶抗体
合格 EZH1 组蛋白赖氨酸N-甲基EZH1抗体
合格 GCET2 **中心B**细胞相关蛋白2抗体
合格 HOXB2 同源盒蛋白B2抗体
合格 合格 ID1 DNA结合抑制因子1抗体
合格 LOXL2 赖氨酰氧化酶相关蛋白2抗体
合格 OLFM4 抗细胞凋亡蛋白OLFM44抗体