Phospho-Aurora B (Tyr12)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 12552R
英文名称Phospho-Aurora B (Tyr12)
中文名称磷酸化有丝分裂激酶B抗体
别 名Aurora B (phospho Y12); AURKB_HUMAN; Aurora B (phospho T12); AIK2; AIM1; ARK2; AurB; AURKB; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora-B; Aurora/IPL1 related kinase 2; IPL1; Serine/theronine kinase 12; Serine/threonine protein kinase 12; STK1; STK12; STK5.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 神经生物学 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
Phospho-Aurora B (Tyr12)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Sheep,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量39kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Aurora B around the phosphorylation site of Tyr12:WP(p-Y)GR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Aurora B (Tyr12)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Mahogany (MG), originally identified as a protein involved in pigmentation, acts in conjunction with melanocortin receptors to suppress diet-induced obesity. Mahogany contains a single transmembrane domain, and it is expressed in a broad range of tissues, including the hypothalamus and pigment cells. Mutations within the mahogany gene were shown to rescue agouti-lethal-yellow mutant mice from obesity. The extracellular domain of mouse mahogany is the ortholog of the human protein attractin. Attractin (also designated DPPT-L) is a human serum glycoprotein and is a member of the CUB family of cell adhesion and guidance proteins. Attractin is expressed on activated T cells and is released from the cells 48 to 72 hours after activation.
Function:
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
Subcellular Location:
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
Tissue Specificity:
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Post-translational modifications:
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
DISEASE:
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
SWISS:
Q96GD4
Gene ID:
9212
Phospho-Aurora B (Tyr12)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Aurora B (Tyr12)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-STAT5b (Ser731) 磷酸化信号转导和转录激活因子5b抗体
合格 LZTS1 假定肿瘤抑制亮氨酸拉链蛋白1抗体
合格 Ensconsin 上皮细胞微管相关蛋白7抗体
合格 MBNL3 毒蕈碱样蛋白3抗体
合格 合格 APRIL/TNFSF13 肿瘤坏死因子配体超家族成员13抗体
合格 LGALS3BP 可溶性半乳糖凝集素3结合蛋白抗体
合格 MARK2 丝氨酸/苏氨酸蛋白激酶MARK2抗体
合格 合格 phospho-MARK2(Thr596) 磷酸化丝氨酸/苏氨酸蛋白激酶MARK2抗体
合格 DBF4B S期激酶活化蛋白DBF4B抗体
合格 CIAPIN1 细胞因子诱导凋亡抑制因子1
合格 FGF23 成纤维细胞生长因子23抗体
合格 FGF19 成纤维细胞生长因子19抗体
合格 FGF22 成纤维细胞生长因子22抗体
合格 Activin A Receptor Type IC 激活素受体1C抗体
合格 Integrin Alpha V + Beta 6 整合素αVβ6抗体
合格 MCPIP1 单核细胞趋化诱导蛋白1抗体
合格 Robo 轴突导向受体抗体
合格 合格 Robo4 肿瘤血管内皮调节蛋白Robo4抗体
合格 BNIP3L 促凋亡BNIP3L蛋白抗体
合格 BAG3 Bcl2抑凋亡蛋白Bag3抗体
合格 BAG5 BCL2分子伴侣调节蛋白5抗体
合格 Lactoferrin 乳铁蛋白抗体
合格 PHLP 肿瘤抑制基因PHLPP抗体
合格 PRODH 脯氨酸脱氢酶抗体
合格 合格 RRAGA RAS相关GTP结合蛋白A抗体
合格 H Cadherin 心脏钙粘蛋白抗体
合格 K Cadherin 钙粘蛋白6抗体
合格 EMP3 上皮细胞膜蛋白3抗体
合格 ESAM 内皮细胞粘附分子抗体
合格 Galectin 8 半乳糖凝集素8抗体
合格 TAG1 瞬时表达轴索糖蛋白1抗体
合格 ADAM11 去整合素样金属蛋白酶11抗体
合格 合格 ADAM15 去整合素样金属蛋白酶15抗体