Phospho-Bad (Ser118)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5216R
英文名称Phospho-Bad (Ser118)
中文名称磷酸化相关死亡促进因子抗体
别 名Bad (phospho S118); Bad (phospho Ser118); p-Bad (S118);p- Bad (Ser118); p-Bad (phospho Ser118); BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Death Promoter; Bcl-2-like protein 8; Bcl2 antagonist of cell death; BCL2 antagonist of cell death protein; BCL2 associated agonist of cell death; Bcl2 Associated Death Promoter; BCL2 binding component 6; BCL2 binding protein; Bcl2 Like 8 Protein; Bcl2-L-8; BCL2L8; BclXL; Proapoptotic BH3 Only Protein; BAD_HUMAN; Bcl-2-binding component 6.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 神经生物学 信号转导 细胞凋亡 线粒体
抗体来源Rabbit
克隆类型Polyclonal
Phospho-Bad (Ser118)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量22kDa
细胞定位细胞浆 细胞膜 线粒体
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser118:RM(p-S)DE
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Bad (Ser118)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Function:
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Subunit:
Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10. The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins. Interacts with AKT1 and PIM3.
Subcellular Location:
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
Tissue Specificity:
Expressed in a wide variety of tissues.
Post-translational modifications:
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 andSer-134 in response to survival stimuli, which blocks itspro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75promotes heterodimerization with 14-3-3 proteins. This interactionthen facilitates the phosphorylation at Ser-118, a site within theBH3 motif, leading to the release of Bcl-X(L) and the promotion ofcell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blockedby the apoptotic C-terminus cleavage product of PKN2 generated bycaspases-3 activity during apoptosis.
Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
Similarity:
Belongs to the Bcl-2 family.
SWISS:
Q92934
Gene ID:
572
Phospho-Bad (Ser118)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Bad (Ser118)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-HDAC1 (Ser423+Ser421) 磷酸化组蛋白去乙酰化酶1抗体
合格 Seladin 1 24脱氢胆固醇还原酶抗体
合格 phospho-HOMER3 (Thr36) 磷酸化HOMER3蛋白抗体
合格 phospho-IKK gamma(Ser43) 磷酸化KB抑制蛋白激酶γ抗体
合格 phospho-IL-1R1(Tyr496) 磷酸化白介素1受体1抗体
合格 phospho-IL-7Ra (Tyr449) 磷酸化白细胞介素-7受体a抗体
合格 phospho-IRS1 (Ser312) 磷酸化胰岛素受体底物-1抗体
合格 phospho-IKK beta(Ser471) 磷酸化KB抑制蛋白激酶β抗体
合格 Phospho-c-Kit(Ser741) 磷酸化原癌基因c-kit抗体
合格 Phospho-c-Kit(Ser721) 磷酸化原癌基因c-kit抗体
合格 Phospho-LRP6(Thr1479) 磷酸化低密度脂蛋白受体相关蛋白6抗体
合格 phospho-Tau (Ser235) 磷酸化微管相关蛋白抗体
合格 phospho-Tau (Thr212) 磷酸化微管相关蛋白抗体
合格 phospho-Tau (Thr205) 磷酸化微管相关蛋白抗体
合格 phospho-Tau (Ser356) 磷酸化微管相关蛋白抗体
合格 PP1C gamma 蛋白磷酸酶γ1抗体
合格 合格 PPAP2A 磷酸脂磷酸水解酶1抗体
合格 phospho-MEK2 (Thr394) 磷酸化丝裂原活化蛋白激酶激酶2抗体
合格 phospho-MEK5(Ser142) 磷酸化丝裂原活化蛋白激酶激酶5抗体
合格 phospho-MEK5(Ser129) 磷酸化丝裂原活化蛋白激酶激酶5抗体
合格 phospho-MEK5(Ser137) 磷酸化丝裂原活化蛋白激酶激酶5抗体
合格 合格 合格 Phospho-TAK1(Ser192) 磷酸化转化生长因子β活化激酶1
合格 合格 phospho-ILK-1(Thr173) 磷酸化整合素连接激酶1抗体
合格 合格 phospho-IRS1 (Thr176) 磷酸化胰岛素受体底物1抗体
合格 phospho-IGF1R (Tyr980) 磷酸化胰岛素样生长因子1受体抗体
合格 phospho-IGF1R (Tyr1280) 磷酸化胰岛素样生长因子1受体抗体
合格 phospho-IGF1R (Tyr1165 + Tyr1166) 磷酸化胰岛素样生长因子1受体抗体
合格 phospho-ICAM1 (Tyr512) 磷酸化细胞间粘附分子1抗体
合格 合格 phospho-c-Jun (Thr91) 磷酸化原癌基因c-Jun抗体
合格 phospho-Smad3 (Ser213) 磷酸化细胞信号转导分子SMAD3抗体
合格 phospho-c-Jun(Thr239) 磷酸化原癌基因c-Jun抗体
合格 phospho-c-Jun (Thr249) 磷酸化原癌基因c-Jun抗体
合格 合格 phospho-c-Jun(Tyr170) 磷酸化原癌基因c-Jun抗体