phospho-Bax (Ser184)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3010R
英文名称phospho-Bax (Ser184)
中文名称磷酸化Bax抗体
别 名Bax (phospho S184); Bax (phospho Ser184); p-Bax (S184); p-Bax (Ser184); apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4.
JournalPMIDIFApplicationThe Journal of Immunology (2013)242497295.5200WBInternational Journal of Oncology (2016)271217483.0200WBMolecular and Cellular Biochemistry (2014)252458182.3880WB
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
phospho-Bax (Ser184)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量21kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Bax around the phosphorylation site of Ser184:TA(p-S)LT
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Bax (Ser184)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].
Function:
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Subunit:
Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2.
Subcellular Location:
Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
Isoform Beta: Cytoplasm.
Isoform Gamma: Cytoplasm.
Isoform Delta: Cytoplasm (Potential).
Tissue Specificity:
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
Similarity:
Belongs to the Bcl-2 family.
SWISS:
Q07812
Gene ID:
581
phospho-Bax (Ser184)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Bax (Ser184)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-CREB-1(Ser142) 磷酸化CREB-1抗体
合格 合格 phospho-CREB-1(Ser121) 磷酸化CREB-1抗体
合格 phospho-CDKN1A/p21 (Ser130) 磷酸化p21蛋白抗体
合格 phospho-CDKN1B (Thr198) 磷酸化P27抗体/周期素依赖激酶抑制剂抗体
合格 Cyclin E2 周期素E2抗体
合格 phospho-CASC3(Tyr181) 磷酸化肿瘤易感候选基因3抗体
合格 phospho-DAXX (Ser517) 磷酸化Fas相关死亡结构域蛋白抗体
合格 phospho-Desmin (Thr16) 磷酸化结蛋白抗体
合格 phospho-Desmin (Thr76 + Thr77) 磷酸化结蛋白抗体
合格 phospho-E2F1 (Ser337) 磷酸化转录因子E2F-1抗体
合格 phospho-EGFR (Tyr1110) 磷酸化表皮生长因子受体抗体
合格 phospho-EGFR (Ser1142) 磷酸化表皮生长因子受体抗体
合格 phospho-CD95/FAS (Tyr291) 磷酸化载脂蛋白A1抗体
合格 Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) 磷酸化碱性成纤维细胞生长因子受体1/2(Tyr463/Tyr466)抗体
合格 合格 Phospho-mTOR (Tyr144) 磷酸化雷帕霉素靶蛋白抗体
合格 Phospho-RhoA (Ser188) 磷酸化RhoA蛋白抗体
合格 合格 Phospho-mTOR (Thr2446) 磷酸化雷帕霉素靶蛋白抗体
合格 Phospho-ELk1(Thr417) 磷酸化细胞转录因子ELK1抗体
合格 Phospho-Estrogen Receptor alpha (Ser305) 磷酸化雌**受体α抗体
合格 phospho-FXYD1 (Ser83) 磷酸化磷酸神经膜抗体
合格 phospho-FXYD1 (Ser88) 磷酸化磷酸神经膜抗体
合格 合格 phospho-GFAP (Ser8) 磷酸化胶质纤维酸性蛋白抗体
合格 phospho-GABBR1 (Ser923) 磷酸化gamma氨基丁酸B型受体1抗体
合格 Phospho-Histone H2A.X (Tyr143) 磷酸化组蛋白H2AX抗体
合格 phospho-HSF1 (Ser303) 磷酸化热休克因子1抗体
合格 phospho-HSF1 (Ser307) 磷酸化热休克因子1抗体
合格 phospho-HSP70 (Tyr525) 磷酸化热休克蛋白70抗体
合格 phospho-GSK-3 Beta(Ser21+Ser29) 磷酸化糖原合酶激酶3β抗体
合格 phospho-GSK-3 Beta(Ser21) 磷酸化糖原合酶激酶3β抗体
合格 phospho-G3BP1(Ser232) 磷酸化Ras GTP酶活化蛋白结合蛋白1抗体
合格 Phospho-NMDAR2B (Tyr1336) 磷酸化谷氨酸受体2B抗体