Phospho-BLNK(Tyr189)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 9688R
英文名称Phospho-BLNK(Tyr189)
中文名称磷酸化T**细胞连接蛋白抗体
别 名BLNK (phospho Y491); p-BLNK (phospho Y491); B cell adapter containing SH2 domain protein; B cell adapter containing Src homology 2 domain protein; B cell linker; B cell linker protein; B cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker protein; BASH; BASH; Bca; Bca; BLNK; BLNK s; BLNK_HUMAN; Cytoplasmic adapter protein; Ly 57; Ly-57; Ly57; Lymphocyte antigen 57; Lymphocyte antigen-57; Lyw 57; Lyw-57; Lyw57; MGC111051; SLP 65; SLP65; SLP-65; Src homology 2 domain containing leukocyte protein of 65 kDa.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 信号转导 细胞凋亡 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
Phospho-BLNK(Tyr189)抗体交叉反应 Human, Mouse, Rat,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量65kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human BLNK around the phosphorylation site of Tyr189 [EN(p-Y)IH]:EN(p-Y)IH
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-BLNK(Tyr189)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012].
Function:
Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Plays an important role in BCR-induced B-cell apoptosis.
Subunit:
Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2. Interacts through its SH2 domain with CD79A.
Subcellular Location:
Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction.
Tissue Specificity:
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
Post-translational modifications:
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
DISEASE:
Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
Similarity:
Contains 1 SH2 domain.
SWISS:
Q8WV28
Gene ID:
29760
Phospho-BLNK(Tyr189)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-BLNK(Tyr189)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 TREML1 髓系细胞触发受体样转录因子1抗体
合格 TPSB2 肥大细胞类胰蛋白酶β2
合格 合格 GSTP1 谷胱甘肽硫转移酶pi基因/Glutathione S Transferase pi抗体
合格 phospho-IRS1 (Ser307) 磷酸化胰岛素受体底物p-IRS-1/2抗体
合格 p53BP1 p53结合蛋白1抗体
合格 RUNX1 急性髓细胞白血病1蛋白抗体
合格 BLNK B**细胞连接蛋白抗体
合格 Aurora C 有丝分裂激酶B抗体
合格 合格 CRK Crk p38抗体
合格 EIF2AK4/GCN2 蛋白激酶GCN2蛋白抗体
合格 GRB10 生长因子受体结合蛋白10抗体
合格 AMPK alpha 2 腺苷单磷酸活化蛋白激酶α2抗体
合格 LIMK1 单丝氨酸蛋白激酶1抗体
合格 CD44v7 CD44V7抗体
合格 CD44v10 CD44V10抗体
合格 合格 CACNA1G 电压依赖性钙通道Cav3.1抗体
合格 CD44v5 CD44V5抗体
合格 CD44v4 CD44V4抗体
合格 Tap1 ATP结合转运因子1抗体
合格 Claudin 4 紧密连接蛋白4抗体
合格 CRHR1 促肾上腺皮质释放**受体1抗体
合格 合格 CRHR2 促肾上腺皮质释放**受体2抗体
合格 MYL1+3 肌球蛋白轻链1/3抗体
合格 NAT2 N-乙酰基转移酶2抗体
合格 合格 Endo G 核酸内切酶G抗体
合格 Cardiac Troponin T 心肌特异性肌钙蛋白T抗体
合格 CD161/NK1.1 CD161抗体
合格 Jak3 蛋白酪氨酸激酶JAK-3抗体
合格 HDAC4 组蛋白去乙酰化酶4抗体
合格 HDAC6 组蛋白去乙酰化酶6抗体
合格 合格 LPA1 溶血磷脂酸受体蛋白1/EDG2抗体
合格 LPA2 溶血磷脂酸受体蛋白2/EDG4抗体
合格 EDG6 内皮细胞的分化蛋白6抗体
合格 GAB2 接头蛋白Gab 2抗体