phospho-CD95/FAS (Tyr291)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5323R
英文名称phospho-CD95/FAS (Tyr291)
中文名称磷酸化载脂蛋白A1抗体
别 名CD95 (phospho Y291); CD95 (phospho Tyr291); p-CD95 (Tyr291); FAS (phospho Y291); FAS (phospho Tyr291); p-FAS (Tyr291); ALPS 1A; ALPS1A; APO 1; Apo 1 antigen; APO 1 cell surface antigen; Apo-1 antigen; APO1; Apo1 antigen; APO1 cell surface antigen; Apoptosis antigen 1; Apoptosis mediating surface antigen FAS; Apoptosis-mediating surface antigen FAS; APT 1; APT1; CD 95; CD 95 antigen; CD95; CD95 antigen; Delta Fas; Delta Fas/APO 1/CD95; Delta Fas/APO1/CD95; FAS 1; FAS 827dupA; Fas AMA; FAS; FAS Antigen; FAS1; FASLG receptor; FASTM; TNF receptor superfamily, member 6; TNFRSF 6; TNFRSF6; TNR6_HUMAN; Tumor necrosis factor receptor superfamily member 6.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
phospho-CD95/FAS (Tyr291)抗体交叉反应 Human,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量34kDa
细胞定位细胞膜 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human FAS around the phosphorylation site of Tyr291:EA(p-Y)DT
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-CD95/FAS (Tyr291)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
FAS is a receptor for TNFSF6/FASL. The adaptor molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kDa, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). It is type I membrane protein. Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adaptor proteins Contains 1 death domain.
Function:
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Subunit:
Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2, 3, 4, 5, 6: Secreted.
Tissue Specificity:
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Post-translational modifications:
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
DISEASE:
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Similarity:
Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
SWISS:
P25445
Gene ID:
355
phospho-CD95/FAS (Tyr291)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-CD95/FAS (Tyr291)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
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合格 合格 TLR1 Toll样受体1抗体
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合格 TOP2A DNA拓普西异构酶ⅡA抗体
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合格 TRF2 端粒结合蛋白2抗体
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合格 CHRNG 烟碱型乙酰胆碱受体γ抗体
合格 NIF1 锌指蛋白335抗体
合格 NIPAL4 镁转运蛋白NIPAL4抗体
合格 NISCH 新型抑癌蛋白Nischarin抗体
合格 NKAIN1 NKAIN1蛋白抗体
合格 UCP-1 线粒体脱偶连蛋白1抗体
合格 NKD2 NKD2蛋白抗体
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合格 ZIK1 锌指蛋白762抗体
合格 NKPD1 NKPD1蛋白抗体
合格 合格 UCP-2 线粒体脱偶连蛋白2抗体
合格 Nkx2.4 转录因子同源蛋白NKX2.4抗体
合格 Nkx2.6 转录因子同源蛋白Nkx2.6抗体
合格 NLRP11 NLRP11蛋白抗体
合格 NLRP13 NLRP13蛋白抗体
合格 NLRP14 NLRP14蛋白抗体
合格 NMNAT2 烟酰胺核苷酸腺苷转移酶2抗体
合格 NMT55 核RNA结合蛋白P54抗体
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合格 NOXA1 NADPH氧化酶激活蛋白1抗体
合格 NPAS2 神经细胞PAS结构域蛋白2抗体
合格 NPFF2 神经肽G蛋白偶联受体抗体
合格 NPIPL2 NPIPL2蛋白抗体
合格 NPL/N-acetylneuraminate lyase 唾液酸醛缩酶NPL抗体
合格 合格 NPRL3 氮酶调节因子3样蛋白抗体
合格 WNT9A 信号通路Wnt9a抗体
合格 NRAP 伴肌动蛋白相关锚定蛋白抗体