phospho-c-Jun (Thr249)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5462R
英文名称phospho-c-Jun (Thr249)
中文名称磷酸化原癌基因c-Jun抗体
别 名c-Jun (Phospho-Thr249); c-Jun (phospho T249); p-c-Jun (phospho T249); Transcription factor AP-1; Jun oncogene; JUN; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; JUN_HUMAN; Activator 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-c-Jun (Thr249)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human c-Jun around the phosphorylation site of Thr249:ME(p-S)QE
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-c-Jun (Thr249)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Function:
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Subunit:
Heterodimer with either FOS or BATF3 or ATF7. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA (By similarity). Interacts with HIVEP3 and MYBBP1A (By similarity). Interacts with SP1, SPIB and TCF20. Interacts with COPS5; the interaction leads indirectly to its phosphorylation. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex. Interacts with RNF187. Binds to HIPK3.
Acetylated at Lys-271 by EP300.
Similarity:
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
SWISS:
P05412
Gene ID:
3725
phospho-c-Jun (Thr249)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-c-Jun (Thr249)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Antithrombin III /SERPINC1 抗凝血酶3抗体
合格 LASP1 LASP1蛋白抗体
合格 GW182 GW182蛋白抗体
合格 SMAD1 细胞信号转导分子Smad-1抗体
合格 hair cortex Cytokeratin 毛皮质细胞角蛋白/细胞角蛋白40抗体
合格 ZZZ3 锌指蛋白ZZZ3抗体
合格 ZYG11BL ZYG11BL蛋白抗体
合格 合格 CD59 CD59抗体
合格 ZUFSP ZUFSP蛋白抗体
合格 ZSWIM4 ZSWIM4蛋白抗体
合格 合格 phospho-FAK(Tyr577) 磷酸化粘着斑激酶抗体
合格 ZSCAN2/ZNF854 锌指蛋白854抗体
合格 ZRANB2/ZNF265 锌指蛋白265抗体
合格 合格 ZRANB3 ZRANB3蛋白抗体
合格 phospho-JNK1 + 2 + 3 (Thr183+Tyr185) 磷酸化氨基末端激酶1/2/3抗体
合格 ZPLD1 ZPLD1蛋白抗体
合格 合格 ZRANB2 锌指蛋白ZNF265抗体
合格 ZNHIT2 ZNHIT2蛋白抗体
合格 合格 phospho-GAP43 (Ser41) 磷酸化神经生长相关蛋白43抗体
合格 ZNF862 锌指蛋白862抗体
合格 ZNF92 锌指蛋白92抗体
合格 phospho-FAK(Ser732) 磷酸化粘着斑激酶抗体
合格 ZNF845 锌指蛋白845抗体
合格 ZNF860 锌指蛋白860抗体
合格 Phospho-Estrogen Receptor alpha (Tyr537) 磷酸化雌**受体α抗体
合格 ZNF804B 锌指蛋白804B抗体
合格 ZNF813 锌指蛋白813抗体
合格 ZNF816A 锌指蛋白816A抗体
合格 ZNF793 锌指蛋白793抗体
合格 ZNF799 锌指蛋白799抗体