phospho-CREB-1(Ser121)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5270R
英文名称phospho-CREB-1(Ser121)
中文名称磷酸化CREB-1抗体
别 名CREB(Phospho-Ser121); p-CREB-1(Ser121); pCREB-1(Ser121); Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-CREB-1(Ser121)抗体交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量37kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser121 [ TD(p-S)QK]:TD(p-S)QK
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-CREB-1(Ser121)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF-2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator.
Function:
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Subunit:
Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity (By similarity). Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1.
Subcellular Location:
Nucleus.
Post-translational modifications:
Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
DISEASE:
Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
Note=A CREB1 mutation has been found in a patient with multiple congenital anomalies consisting of agenesis of the corpus callosum, cerebellar hypoplasia, severe neonatal respiratory distress refractory to surfactant, thymus hypoplasia, and thyroid follicular hypoplasia (PubMed:22267179).
Similarity:
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
SWISS:
P16220
Gene ID:
1385
phospho-CREB-1(Ser121)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-CREB-1(Ser121)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Epsin 2 内吞作用辅助蛋白EPN2抗体
合格 Integrin Alpha V + Beta 3 (CD51+CD61) 整合素αVβ3抗体
合格 ETL EGF**受体7跨膜结构域蛋白1抗体
合格 ETV3L ETV3L蛋白抗体
合格 EXOC2 胞吐囊复合体蛋白2抗体
合格 Exonuclease 1 核酸外切酶1抗体
合格 EXOSC10 多发性肌炎/硬皮病自身抗原2抗体
合格 EXOSC7 核糖体RNA合成蛋白42抗体
合格 Factor I light chain 补体因子I轻链抗体
合格 FAM134B FAM134B蛋白抗体
合格 phospho-FANCA (Ser1149) 磷酸化范可尼贫血组蛋白A抗体
合格 phospho-FANCD2(Ser1404) 磷酸化FANCD2抗体
合格 TRRAP 转录因子相关蛋白TRRAP抗体
合格 Oxytocin R 催产素受体(缩宫素受体)抗体
合格 合格 FCGRT IgG-Fc片断受体转运蛋白α抗体
合格 FHOD3 FHOD3蛋白抗体
合格 Nogo A+B 轴索过度生长抑制因子A+B抗体 Nogo-B/A
合格 Ficolin 2 弹性蛋白结合蛋白Fcn2抗体
合格 FBX09 F-box蛋白9抗体
合格 FENS1 磷酸肌醇结合蛋白1抗体
合格 PDGFBB 血小板源性生长因子BB抗体
合格 FIZ1 锌指蛋白798抗体
合格 FKBP10 肽基脯氨酰顺反异构酶FKBP10抗体
合格 STAT1 信号转导与转录激活因子1抗体
合格 FLNC 细丝蛋白2抗体
合格 phospho-FLNC (2233) 磷酸化细丝蛋白2抗体
合格 合格 IL-5 白细胞介素5抗体
合格 FNDC3A Ⅲ型纤维连接蛋白域蛋白3A抗体
合格 Folate Receptor 4 叶酸受体4抗体
合格 phospho-Fos B (Ser27) 磷酸化癌基因FOS蛋白B抗体
合格 FOXD2 叉头蛋白D2抗体
合格 MAPK4 丝裂原活化蛋白激酶4抗体
合格 phospho-FOXO4 (Thr451) 磷酸化叉头蛋白4抗体
合格 FOXRED1 单跨膜蛋白FOXRED1抗体