phospho-DDIT3 (Ser30)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5177R
英文名称phospho-DDIT3 (Ser30)
中文名称磷酸化GADD153抗体
别 名DDIT3 (phospho S30); DDIT3 (phospho Ser30); DDIT3 (phospho-Ser30); p-DDIT3 (phospho S30); ; GADD153; CHOP; Growth arrest and DNA damage-inducible 153; C/EBP homologous protein; C/EBP Homology Protein; CEBPZ; CHOP10; DDIT 3; DNA Damage Inducible Transcript 3; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153; Growth arrest and DNA damage inducible protein GADD153; MGC4154; DDIT3_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域**学 信号转导 细胞凋亡 细胞周期蛋白 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-DDIT3 (Ser30)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量19kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human GADD153 around the phosphorylation site of Ser30:VL(p-S)SD
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-DDIT3 (Ser30)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Function:
Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.
Subunit:
Heterodimer. Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6. Interacts with TRIB3 which blocks its association with EP300/P300. Interacts with FOXO3, CEBPB and ATF4.
Subcellular Location:
Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation.
Tissue Specificity:
Ubiquitinated, leading to its degradation by the proteasome.
Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity
Post-translational modifications:
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
Similarity:
Belongs to the bZIP family.
Contains 1 bZIP (basic-leucine zipper) domain.
SWISS:
P35638
Gene ID:
1649
phospho-DDIT3 (Ser30)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-DDIT3 (Ser30)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 FAM98A FAM98A蛋白抗体
合格 ILDR1 **球蛋白样结构域受体1抗体
合格 合格 ILVBL 乙酰乳酸合成酶蛋白抗体
合格 GFAP delta 胶质纤维酸性蛋白GFAPδ抗体
合格 CD276 CD276抗体
合格 Gigaxonin 巨轴索神经病蛋白GAN抗体
合格 合格 Kinesin/KIF5B 驱动蛋白KNS1抗体
合格 RP1 视网膜色素变性蛋白1抗体
合格 Gliomedin 神经胶质蛋白(肝癌相关基因2)抗体
合格 PD-L1 程序性死亡配体1抗体
合格 C1QL1 补体组分1q子成分样蛋白1抗体
合格 Elastin 项韧带弹性蛋白抗体
合格 Caspase-12 半胱胺酸蛋白酶蛋白-12抗体
合格 procollagen type IIA Ⅱ型胶原α1 N端前体肽抗体
合格 CDH18 钙粘附分子18抗体
合格 CNTN4/AXCAM 轴突相关粘附分子抗体
合格 PTHLH 甲状旁腺**相关蛋白抗体
合格 合格 JAMC 连接粘附分子C抗体
合格 Myelin PLP 髓磷酯髓鞘蛋白1抗体
合格 NCAM2 神经细胞粘附分子2抗体
合格 NLGN2 神经连接蛋白2抗体
合格 PCDH12 原钙粘附蛋白12/血管内皮钙粘蛋白2抗体
合格 合格 合格 Osterix 成骨相关转录因子抗体
合格 合格 PCDH18 原钙粘附蛋白18抗体
合格 PCDH20 原钙粘附蛋白20抗体
合格 PCDH8 原钙粘附蛋白8抗体
合格 PCDHA1 原钙粘附蛋白α1抗体
合格 PCDHA9 原钙粘附蛋白α9抗体
合格 合格 PCDHA8 原钙粘附蛋白α8抗体
合格 PCDHA11 原钙粘蛋白11抗体
合格 CD11b 整合素αM抗体
合格 PCDHAC2 原钙粘蛋白介导的PCDHαC2受体抗体
合格 IL-28R 白细胞介素28受体抗体
合格 CD5 CD5抗体
合格 PCDHB12 原钙粘蛋白β12抗体
合格 PCDHGB1 原钙粘蛋白γB1抗体
合格 AMPK alpha 1 腺苷单磷酸活化蛋白激酶α1抗体
合格 SorLA 低密度脂蛋白受体相关蛋白9抗体
合格 合格 GPR49 G蛋白偶联受体49抗体