phospho-EphB1 (Tyr928)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 13082R
英文名称phospho-EphB1 (Tyr928)
中文名称磷酸化酪氨酸蛋白激酶受体B1抗体
别 名Eph receptor B1 (phospho Tyr928); Eph receptor B1(phospho Y928); p-EphB1(Y928); p-EphB1(Tyr928); Cek 6; Elkh; Eph tyrosine kinase 2; EPH2; EphB1; Ephrin receptor Eph B1 precursor; Ephrin type B receptor 1; HEK 6; NET; Neuronally expressed EPH related tyrosine kinase; Tyrosine protein kinase receptor EPH 2; EPHB1_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域心血管 细胞生物 神经生物学 信号转导 激酶和磷酸酶 细胞膜受体
抗体来源Rabbit
克隆类型Polyclonal
phospho-EphB1 (Tyr928)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Zebrafish, Sheep, Xenopus laevis
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量108kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human Eph receptor B1 around the phosphorylation site of Tyr928:VQ(p-Y)RD
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-EphB1 (Tyr928)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]
Function:
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the ***** nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Subunit : Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1. Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion. The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (residues within the catalytic domain) with GRB2 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1
Subunit:
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1 (By similarity). Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion (By similarity). The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (residues within the catalytic domain) with GRB2 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, dendrite.
Tissue Specificity:
Preferentially expressed in brain.
Post-translational modifications:
Phosphorylated. Autophosphorylation is stimulated by the ligand EFNB1. Required for interaction with SH2 domain-containing interactors, for activation of the MAPK/ERK and JUN signaling cascades and for ubiquitination by CBL.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
SWISS:
P54762
Gene ID:
2047
phospho-EphB1 (Tyr928)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-EphB1 (Tyr928)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 CD36 CD36抗体
合格 LC3 自噬微管相关蛋白轻链3抗体
合格 Cyclin E1 周期素E1抗体
合格 合格 Connexin 43 间隙连接蛋白43抗体
合格 合格 IFN-Alpha 干扰素α抗体
合格 合格 CD34 CD34抗体
合格 合格 Annexin VI 膜粘连蛋白6抗体
合格 CECR1 猫眼综合征染色体候选基因1抗体
合格 CECR6 猫眼综合征染色体候选基因6抗体
合格 EFHD1 EFHD1蛋白抗体
合格 IQCG IQCG蛋白抗体
合格 AKAP2/PALM2 蛋白激酶A2抗体
合格 TRPM6 瞬时受体电位离子通道蛋白6抗体（M亚家族）
合格 TRPM4 瞬时受体电位离子通道蛋白4抗体（M亚家族）
合格 DIS3L 有丝分裂控制样蛋白DIS3L抗体
合格 Dermokine beta Dermokine β蛋白抗体
合格 hnRNP A2B1 异质核糖核蛋白hnRNPA2抗体
合格 合格 AGXT2L2 AGXT2L2蛋白抗体
合格 AER61 未知糖基化转移酶AER61抗体
合格 ARSK 芳香基硫酸酯酶K抗体
合格 ART3 二磷酸腺苷核糖基转移酶3抗体
合格 ASMTL ASMTL蛋白抗体
合格 Neurexin-3-beta 轴突蛋白3 beta抗体
合格 COPS8 信号转导蛋白亚基8抗体
合格 ASB17 含锚蛋白重复序列-细胞因子信号抑制物盒蛋白家族17抗体
合格 ZSWIM3 ZSWIM3蛋白抗体
合格 UBOX5 泛素结合酶7相互作用蛋白5抗体
合格 TTC33 TTC33蛋白抗体
合格 RNF92/TRIM11 环指蛋白92抗体
合格 TRIM41 环指蛋白调节蛋白激酶C蛋白抗体
合格 TRIM50 TRIM50蛋白抗体
合格 RNF142 环指蛋白142抗体
合格 合格 RNF93 环指蛋白93抗体
合格 合格 RNF14 环指蛋白14抗体
合格 RNF15 环指蛋白15抗体