ERK1 (pT202/pY204) + Erk2 (pT185/pY187)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 14624R
英文名称ERK1 (pT202/pY204) + Erk2 (pT185/pY187)
中文名称磷酸化丝裂原活化蛋白激酶1/2抗体
别 名ERK 1; ERK 2; ERK; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen activated protein kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_HUMAN; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44MAPK; PRKM 1; PRKM 2; PRKM 3; PRKM1; PRKM2; PRKM3; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 3; Protein tyrosine kinase ERK 2.
说 明 书100ul
产品类型磷酸化抗体
研究领域细胞生物 神经生物学 信号转导 Alzheimer's
抗体来源Rabbit
克隆类型Polyclonal
ERK1 (pT202/pY204) + Erk2 (pT185/pY187)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Xenopus laevis
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量41kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ERK1/ERK2 around the phosphorylation site of ERK1(T202 + Y204) or Erk2 (T185 + Y187):FL(P-T)E(P-Y)VA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
ERK1 (pT202/pY204) + Erk2 (pT185/pY187)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jul 2008]
Function:
Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. MAPK1/ERK2and MAPK3/ERK1 are the 2 MAPKs which play an important role in theMAPK/ERK cascade. They participate also in a signaling cascadeinitiated by activated KIT and KITLG/SCF. Depending on the cellularcontext, the MAPK/ERK cascade mediates diverse biological functionssuch as cell growth, adhesion, survival and differentiation throughthe regulation of transcription, translation, cytoskeletalrearrangements. The MAPK/ERK cascade plays also a role ininitiation and regulation of meiosis, mitosis, and postmitoticfunctions in differentiated cells by phosphorylating a number oftranscription factors. About 160 substrates have already beendiscovered for ERKs. Many of these substrates are localized in thenucleus, and seem to participate in the regulation of transcriptionupon stimulation. However, other substrates are found in thecytosol as well as in other cellular organelles, and those areresponsible for processes such as translation, mitosis andapoptosis. Moreover, the MAPK/ERK cascade is also involved in theregulation of the endosomal dynamics, including lysosome processingand endosome cycling through the perinuclear recycling compartment(PNRC); as well as in the fragmentation of the Golgi apparatusduring mitosis. The substrates include transcription factors (suchas ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements(such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1),regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3,MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and avariety of other signaling-related molecules (like ARHGEF2, DCC,FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1,RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1,MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) andphosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are othersubstrates which enable the propagation the MAPK/ERK signal toadditional cytosolic and nuclear targets, thereby extending thespecificity of the cascade. Mediates phosphorylation of TPR inrespons to EGF stimulation. May play a role in the spindle assemblycheckpoint. Phosphorylates PML and promotes its interaction withPIN1, leading to PML degradation (By similarity). [FUNCTION] Acts as a transcriptional repressor. Binds to a[GC]AAA[GC] consensus sequence. Repress the expression ofinterferon gamma-induced genes. Seems to bind to the promoter ofCCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 andSTAT1. Transcriptional activity is independent of kinase activity(By similarity).
Subunit:
Binds both upstream activators and downstream substratesin multimolecular complexes. Interacts with ADAM15, ARHGEF2, ARRB2,DAPK1 (via death domain), HSF4, IER3, IPO7, DUSP6, NISCH, SGK1, andisoform 1 of NEK2. Interacts (via phosphorylated form) with TPR(via C-terminus region and phosphorylated form); the interactionrequires dimerization of MAPK1/ERK2 and increases following EGFstimulation (By similarity). Interacts (phosphorylated form) withCAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted byinsulin, leads to nuclear location and MAPK1 activation (Bysimilarity). Interacts with DCC (By similarity). Interacts withMORG1, PEA15 and MKNK2. MKNK2 isoform 1 binding prevents fromdephosphorylation and inactivation. The phosphorylated forminteracts with PML (By similarity).
Subcellular Location:
Cytoplasm, cytoskeleton, spindle (Bysimilarity). Nucleus. Cytoplasm, cytoskeleton, centrosome (Bysimilarity). Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase (By similarity). PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-244 and Ser-246 as well asautophosphorylation at Thr-188 promote nuclear localization (Bysimilarity).
Tissue Specificity:
Widely expressed.
Post-translational modifications:
Dually phosphorylated on Thr-183 and Tyr-185, which activatesthe enzyme. Ligand-activated ALK induces tyrosine phosphorylation(By similarity). Dephosphorylated by PTPRJ at Tyr-185 (Bysimilarity). Phosphorylated upon FLT3 and KIT signaling (Bysimilarity).
Similarity:
Belongs to the protein kinase superfamily. CMGCSer/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Gene ID:
5595
ERK1 (pT202/pY204) + Erk2 (pT185/pY187)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
ERK1 (pT202/pY204) + Erk2 (pT185/pY187)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 phospho-C-Myc(Ser373) 磷酸化致癌基因C-Myc抗体
合格 FNDC1 Ⅲ型纤维连接蛋白域蛋白1抗体
合格 MX2 + MX1 干扰素诱导GTP结合蛋白MX2抗体
合格 APLP2 淀粉样蛋白β前体样蛋白2抗体
合格 合格 APLP1 淀粉样蛋白β前体样蛋白1抗体
合格 FBXL17 FBXL17蛋白抗体
合格 合格 FBXO36 FBXO36 F-box蛋白相关蛋白36抗体
合格 FBXO33 F-box蛋白相关蛋白33抗体
合格 FBXL15 FBXL15蛋白抗体
合格 MST1/MST2 蛋白激酶MST1/2抗体
合格 NMDA epsilon 2 谷氨酸受体NMDAζ2抗体
合格 FBXO18 F-box蛋白家族FBXO18抗体
合格 RNF211 环指蛋白211抗体
合格 合格 caspase-9 p10 半胱胺酸蛋白酶蛋白9-p10抗体
合格 hnRNP M 异质性核糖核蛋白M抗体
合格 OSTM1 骨硬化病相关跨膜蛋白1抗体
合格 Reg3a precursor 胰岛β细胞生长因子抗体
合格 合格 MSX1 MSH同源蛋白1样蛋白抗体
合格 MtTF1/TFAM 线粒体转录因子1抗体
合格 Calcium Sensing Receptor 钙敏感受体1抗体
合格 合格 TPP1 细胞生长抑制基因1蛋白抗体
合格 合格 Vimentin 波形蛋白抗体
合格 合格 TRPV5 辣椒素受体5抗体
合格 合格 Nucleolin/C23 核仁蛋白C23抗体
合格 EP4 前列腺素E受体蛋白4抗体
合格 CLEC 4E C型凝集素4家族E抗体
合格 合格 GABR B3 G氨基丁酸受体3抗体
合格 TRAF4 肿瘤坏死因子受体相关蛋白4抗体
合格 合格 3-Nitrotyrosine 硝基酪氨酸抗体
合格 Desmuslin 中间丝蛋白β-synemin抗体
合格 non-muscle Myosin IIA 非平滑肌肌球蛋白2A抗体
合格 Phospho-NMDAR2B (Tyr1474) 磷酸化谷氨酸受体2B抗体
合格 FSIP1 纤维性鞘结合蛋白1抗体
合格 Arginase 1 精氨酸酶1抗体
合格 DOK5 胞浆接头蛋白Dok5抗体
合格 Etv5 转录因子Ets差异基因5抗体
合格 phospho-PKC Epsilon(Ser729) 磷酸化蛋白激酶C/p-PKC ε抗体
合格 LGR6 G蛋白偶联受体6抗体