phospho-ERK5 ( Ser731+Thr733)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5486R
英文名称phospho-ERK5 ( Ser731+Thr733)
中文名称磷酸化细胞外信号调节激酶5抗体
别 名ERK5 (phospho Ser731 + Thr733); Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; EC 2.7.11.24; ERK 4; ERK 5; ERK-5; ERK4; Extracellular Signal Regulated Kinase 5; Extracellular signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen Activated Protein Kinase 7; Mitogen-activated protein kinase 7; MK07_HUMAN; OTTHUMP00000065906; OTTHUMP00000065907; PRKM 7; PRKM7; PROTEIN KINASE, MITOGEN-ACTIVATED, 7.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 信号转导 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-ERK5 ( Ser731+Thr733)抗体交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量90kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ERK5 around the phosphorylation site of Ser731+Thr733:VF(p-S)G(p-T)PK
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ERK5 ( Ser731+Thr733)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function:
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression.
Subunit:
Interacts with MAP2K5. Forms oligomers. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D. Interacts with SGK1.
Subcellular Location:
Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation.
Tissue Specificity:
Expressed in many tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
Post-translational modifications:
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
Q13164
Gene ID:
5598
phospho-ERK5 ( Ser731+Thr733)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ERK5 ( Ser731+Thr733)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 CCDC46 卷曲螺旋结构域蛋白46抗体
合格 CCDC47 卷曲螺旋结构域蛋白47抗体
合格 CCDC54 卷曲螺旋结构域蛋白54抗体
合格 合格 CCDC83 卷曲螺旋结构域蛋白83抗体
合格 CCDC87 卷曲螺旋结构域蛋白87抗体
合格 FAM36A FAM36A蛋白抗体
合格 FAM134C FAM134C蛋白抗体
合格 CHD3 ATP依赖的解旋酶CHD3抗体
合格 RB1CC1/FIP200 RB1的诱导卷曲蛋白RB1CC1抗体
合格 CHCHD6 卷曲螺旋结构域蛋白CHCHD6抗体
合格 PHF8 组蛋白赖氨酸去甲基化酶PHF8抗体
合格 KIF11 驱动蛋白样蛋白1抗体（甲状腺**受体相互作用蛋白5）
合格 合格 COMMD9 铜代谢结构域蛋白9抗体
合格 合格 COMMD8 铜代谢结构域蛋白8抗体
合格 COMMD3 铜代谢结构域蛋白3抗体
合格 AVSD2 富含半胱氨酸与表皮生长因子样蛋白2抗体
合格 合格 CRELD2 富含半胱氨酸与表皮生长因子样蛋白2抗体
合格 合格 BRN4 脑转录因子4蛋白抗体
合格 Nestin 巢蛋白/神经上皮干细胞蛋白抗体
合格 合格 FAM113A FAM113A蛋白抗体
合格 FAM13C1 FAM13C1蛋白抗体
合格 FAM59A FAM59A蛋白抗体
合格 FAM151A FAM151A蛋白抗体
合格 FAM153B FAM153B蛋白抗体
合格 FAM154A FAM154A蛋白抗体
合格 FAM155A FAM155A蛋白抗体
合格 FAM160B1 FAM160B1蛋白抗体
合格 FAM161B FAM161B蛋白抗体
合格 FAM164B FAM164B蛋白抗体
合格 FAM101A FAM101A蛋白抗体
合格 FRMD1 FRMD1蛋白抗体
合格 CTDSPL2 CTDSPL2蛋白抗体
合格 DEPTOR DEPTOR蛋白抗体
合格 TBX1 先心病相关蛋白TBX1抗体
合格 DHRS13 短链脱氢还原酶13抗体
合格 DHRS7 视网膜短链脱氢酶/还原酶家族7抗体