Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5326R
英文名称Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)
中文名称磷酸化碱性成纤维细胞生长因子受体1/2(Tyr463/Tyr466)抗体
别 名FGFR1 + FGFR2 (Phospho Tyr463/Tyr466); FGFR1 + FGFR2 (Phospho Tyr463+Tyr466); FGFR2 (Phospho Y463+Y466); bFGF R; BFGFR; C FGR; CD 331; CD331; CD331 antigen; CEK; FGFBR; FGFR 1; Fibroblast growth factor receptor 1; FLG; FLG protein; FLJ14326; FLT 2; FLT2; Fms like tyrosine kinase 2; Fms related tyrosine kinase 2; Fms related tyrosine kinase 2 Pfeiffer syndrome; H2; H3; H4; H5; HBGFR; Heparin binding growth factor receptor; Hydroxyaryl protein kinase; KAL 2; KAL2; MFR; N SAM; N sam tyrosine kinase; Protein tyrosine kinase; Tyrosylprotein kinase; Basic fibroblast growth factor receptor 1.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 生长因子和** 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)抗体交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量88kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr463:SE(p-Y)EL <Cytoplasmic>
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.
Function:
Receptor for fibroblast growth factors FGF2 and FGF1. Receptor for FGF23 in the presence of KL (By similarity). Promotes mitogenesis in response to fibroblast growth factors. Activates PLCG1.
Subunit:
Homodimer. Interacts with KLB. Interacts with KL and FGF23. Interacts with SHB and GRB10. Interacts with PLCG1 (via SH2 domains). Interacts with KAL1; this interaction does not interfere with FGF2-binding to FGFR1, but prevents binding of heparin-bound FGF2. Interacts with SOX2 and SOX3.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
Post-translational modifications:
Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
DISEASE:
Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
SWISS:
P11362
Gene ID:
2260
Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-CDKN1A/p21 (Ser146) 磷酸化p21蛋白抗体
合格 Phospho-Caspase-9 (Ser196) 磷酸化半胱氨酸蛋白酶9抗体
合格 phospho-CREB-1(Ser142) 磷酸化CREB-1抗体
合格 合格 phospho-CREB-1(Ser121) 磷酸化CREB-1抗体
合格 phospho-CDKN1A/p21 (Ser130) 磷酸化p21蛋白抗体
合格 phospho-CDKN1B (Thr198) 磷酸化P27抗体/周期素依赖激酶抑制剂抗体
合格 Cyclin E2 周期素E2抗体
合格 phospho-CASC3(Tyr181) 磷酸化肿瘤易感候选基因3抗体
合格 phospho-DAXX (Ser517) 磷酸化Fas相关死亡结构域蛋白抗体
合格 phospho-Desmin (Thr16) 磷酸化结蛋白抗体
合格 phospho-Desmin (Thr76 + Thr77) 磷酸化结蛋白抗体
合格 phospho-E2F1 (Ser337) 磷酸化转录因子E2F-1抗体
合格 phospho-EGFR (Tyr1110) 磷酸化表皮生长因子受体抗体
合格 phospho-EGFR (Ser1142) 磷酸化表皮生长因子受体抗体
合格 phospho-CD95/FAS (Tyr291) 磷酸化载脂蛋白A1抗体
合格 Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) 磷酸化碱性成纤维细胞生长因子受体1/2(Tyr463/Tyr466)抗体
合格 合格 Phospho-mTOR (Tyr144) 磷酸化雷帕霉素靶蛋白抗体
合格 Phospho-RhoA (Ser188) 磷酸化RhoA蛋白抗体
合格 合格 Phospho-mTOR (Thr2446) 磷酸化雷帕霉素靶蛋白抗体
合格 Phospho-ELk1(Thr417) 磷酸化细胞转录因子ELK1抗体
合格 Phospho-Estrogen Receptor alpha (Ser305) 磷酸化雌**受体α抗体
合格 phospho-FXYD1 (Ser83) 磷酸化磷酸神经膜抗体
合格 phospho-FXYD1 (Ser88) 磷酸化磷酸神经膜抗体
合格 合格 phospho-GFAP (Ser8) 磷酸化胶质纤维酸性蛋白抗体
合格 phospho-GABBR1 (Ser923) 磷酸化gamma氨基丁酸B型受体1抗体
合格 Phospho-Histone H2A.X (Tyr143) 磷酸化组蛋白H2AX抗体
合格 phospho-HSF1 (Ser303) 磷酸化热休克因子1抗体
合格 phospho-HSF1 (Ser307) 磷酸化热休克因子1抗体
合格 phospho-HSP70 (Tyr525) 磷酸化热休克蛋白70抗体