Phospho-Histone H3 (Ser28)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3187R
英文名称Phospho-Histone H3 (Ser28)
中文名称磷酸化组蛋白H3抗体
别 名Histone H3 (phospho S28); p-Histone H3 (phospho S28); Phospho-Histone H3(Ser28); Histone H3(Phospho-Ser28); Histone H3(p-Ser28); Histone H3.3 (phospho S28); Histone H3(Phospho-S28); H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_TETTH; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 染色质和核信号 信号转导 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
Phospho-Histone H3 (Ser28)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit, Fruit Fly,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量15kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser28:RK(p-S)AP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Histone H3 (Ser28)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Function:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.
Subunit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases histone-protein interactions. Interacts with PDD1 and PDD3.
Subcellular Location:
Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC).
Post-translational modifications:
Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or meiotic micronuclear divisions.
Acetylation of histone H3 leads to transcriptional activation. H3K14ac formation by GCN5 is promoted by H3S10ph. H3K9acK14ac is the preferred acetylated form of newly synthesized H3. Acetylation occurs almost exclusively in the MAC.
Methylated to form H3K4me. H3K4me is only found in the transcriptionally active MAC. Methylated to form H3K9me in developing MACs during conjugation, when genome-wide DNA elimination occurs. At this stage, H3K9me specifically occurs on DNA sequences being eliminated (IES), probably targeted by small scan RNAs (scnRNAs) bound to IES, and is required for efficient IES elimination. H3K9me is required for the interaction with the chromodomains of PDD1 and PDD3.
The full-length protein H3S (slow migrating) is converted to H3F (fast migrating) by proteolytic removal of the first 6 residues. H3F is unique to MIC, and processing seems to occur regularly each generation at a specific point in the cell cycle.
Similarity:
Belongs to the histone H3 family.
SWISS:
Q16695
Gene ID:
8290
Phospho-Histone H3 (Ser28)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Histone H3 (Ser28)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-SMAD5 (Ser463 + Ser465) 磷酸化细胞信号转导分子SMAD5抗体
合格 phospho-SMC1 (Ser966) 磷酸化染色体结构维持蛋白质１抗体
合格 MTOR 雷帕霉素靶蛋白抗体
合格 TIMM22 线粒体内膜转位同源蛋白22抗体
合格 TIMM50 线粒体内膜转位同源蛋白50抗体
合格 TIP49A TIP60相关蛋白54α抗体
合格 TIPIN TIMELESS相互作用蛋白抗体
合格 TM2D1 β淀粉样蛋白结合蛋白抗体
合格 TMCO3 跨膜和卷曲螺旋结构域蛋白3抗体
合格 TMEM109 跨膜蛋白109抗体
合格 PRDM5 PR结构域锌指蛋白5抗体
合格 PREX2 磷脂酰肌醇PREX2抗体
合格 PRKRIR 蛋白激酶PKR抑制蛋白PRKRIR抗体
合格 PPP2R2D 蛋白磷酸酶2调节亚基2D抗体
合格 CD171/L1CAM 神经细胞粘附分子配体1抗体
合格 PPP2R5A 蛋白磷酸酶2调节亚基5A抗体
合格 PPP2R5E 蛋白磷酸酶2调节亚基5E抗体
合格 合格 PPP3CC 蛋白磷酸酶3调节亚基C抗体
合格 PPP3R2 蛋白磷酸酶3调节亚基R2抗体
合格 PPP1R16B 蛋白磷酸酶1调节亚基抑制蛋白16B抗体
合格 合格 PABP2 多聚腺苷酸结合蛋白2抗体
合格 PACSIN2 胞浆磷蛋白PACSIN2抗体
合格 合格 RUNX2 核心结合因子α1/成骨特异性转录因子/Cbfα1抗体
合格 Intestinal alkaline phosphatase 肠道碱性磷酸酶抗体
合格 合格 Placental alkaline phosphatase 胎盘碱性磷酸酶抗体
合格 CARD12 凋亡加强结构域蛋白12抗体
合格 PIM3 丝氨酸/苏氨酸蛋白激酶PIM3抗体
合格 phospho-GFAP (Thr7) 磷酸化胶质纤维酸性蛋白抗体
合格 C19orf56 19号染色体开放阅读框56抗体
合格 HSV1 + HSV2 gD 单纯疱疹病毒糖蛋白D抗体
合格 合格 UGT1A7 尿苷二磷酸葡糖醛基转移酶1A7抗体
合格 PCNA 增殖细胞核抗原抗体
合格 LY6G6F **细胞抗原6重复位点蛋白G6F抗体
合格 合格 合格 phospho-CDKN1B (Ser10) 磷酸化P27抗体/周期素依赖激酶抑制剂
合格 合格 Phospho-NFKB1 (Ser373) 磷酸化细胞核因子p50/k基因结合核因子抗体