Phospho-Histone H3 (Ser10)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3186R
英文名称Phospho-Histone H3 (Ser10)
中文名称磷酸化组蛋白H3抗体
别 名Histone H3 (phospho S10); Phospho-Histone H3(Ser10); Histone H3(Phospho-Ser10); Histone H3(p-Ser10); Histone H3(Phospho-S10); H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H31_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 染色质和核信号 信号转导 转录调节因子 细胞类型标志物 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
Phospho-Histone H3 (Ser10)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit, Fruit Fly,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量15kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10:RK(p-S)TG
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Histone H3 (Ser10)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
Function:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.
Subunit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases histone-protein interactions. Interacts with PDD1 and PDD3.
Subcellular Location:
Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC).
Post-translational modifications:
Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or meiotic micronuclear divisions.
Acetylation of histone H3 leads to transcriptional activation. H3K14ac formation by GCN5 is promoted by H3S10ph. H3K9acK14ac is the preferred acetylated form of newly synthesized H3. Acetylation occurs almost exclusively in the MAC.
Methylated to form H3K4me. H3K4me is only found in the transcriptionally active MAC. Methylated to form H3K9me in developing MACs during conjugation, when genome-wide DNA elimination occurs. At this stage, H3K9me specifically occurs on DNA sequences being eliminated (IES), probably targeted by small scan RNAs (scnRNAs) bound to IES, and is required for efficient IES elimination. H3K9me is required for the interaction with the chromodomains of PDD1 and PDD3.
The full-length protein H3S (slow migrating) is converted to H3F (fast migrating) by proteolytic removal of the first 6 residues. H3F is unique to MIC, and processing seems to occur regularly each generation at a specific point in the cell cycle.
Similarity:
Belongs to the histone H3 family.
SWISS:
Q16695
Gene ID:
8290
Phospho-Histone H3 (Ser10)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Histone H3 (Ser10)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 SLC39A14 溶质载体家族蛋白39成员A14抗体
合格 SLC39A2 溶质载体家族蛋白39成员A2抗体
合格 合格 SLC39A4 溶质载体家族蛋白39成员A4抗体
合格 SLC39A5 溶质载体家族蛋白39成员A5抗体
合格 SLC22A1 溶质载体家族蛋白22成员A1/有机阳离子转运蛋白1抗体
合格 RGS13 G蛋白信号转导调节因子13抗体
合格 RGS17 G蛋白信号转导调节因子17抗体
合格 RGS18 G蛋白信号转导调节因子18抗体
合格 RGS3 G蛋白信号转导调节因子3抗体
合格 RGS6 G蛋白信号转导调节因子6抗体
合格 RHBG RH家族B型糖蛋白RHBG抗体
合格 MCP-3 单核细胞趋化蛋白3抗体
合格 合格 Rho guanine exchange factor 16 鸟嘌呤交换因子16抗体
合格 RhoF RhoF蛋白抗体
合格 phospho-Parkin (Ser131) 磷酸化帕金森病蛋白2抗体
合格 PARN 多聚腺苷酸特异性核糖核酸抗体
合格 PARP14 多腺苷二磷酸多聚酶PARP14抗体
合格 PARP8 多腺苷二磷酸多聚酶PARP8抗体
合格 PARVB 小细胞蛋白PARVβ抗体
合格 PARVA 基质重塑相关蛋白PARVA抗体
合格 phospho-Src (Ser75) 磷酸化src原癌基因抗体
合格 phospho-Parvin alpha (Ser8) 磷酸化基质重塑相关蛋白PARVA抗体
合格 PASD1/CT63 癌/睾丸抗原63
合格 合格 PATL2 PATL2蛋白抗体
合格 ARP1B/PC3 肌动蛋白相关蛋白1B抗体
合格 PCBD1 蝶呤甲醇胺脱水酶1抗体
合格 PCBD2 蝶呤甲醇胺脱水酶2抗体
合格 PCBP1 异构核糖核蛋白E1抗体
合格 PCBP2 异构核糖核蛋白E2抗体
合格 PCCA 丙酰辅酶A羧化酶α链抗体
合格 MITF MITF相关转录因子抗体
合格 PCCB 丙酰辅酶A羧化酶β链抗体
合格 PCTAIRE1 周期素依赖性激酶PCTAIRE1抗体