phospho-HSF1 (Ser307)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5364R
英文名称phospho-HSF1 (Ser307)
中文名称磷酸化热休克因子1抗体
别 名HSF1 (phospho S307); p-HSF1 (phospho S307); HSF1 (Phospho-Ser307); Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSTF 1; HSTF1; HSF1_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-HSF1 (Ser307)抗体交叉反应 Human, Mouse,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量57kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303:PQ(p-S)PR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-HSF1 (Ser307)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008].
Function:
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher ukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Subunit:
Monomer. Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2.
Subcellular Location:
Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
Post-translational modifications:
Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat.
Sumoylated by SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
Similarity:
Belongs to the HSF family.
Gene ID:
3297
phospho-HSF1 (Ser307)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-HSF1 (Ser307)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 NLRP13 NLRP13蛋白抗体
合格 NLRP14 NLRP14蛋白抗体
合格 NMNAT2 烟酰胺核苷酸腺苷转移酶2抗体
合格 NMT55 核RNA结合蛋白P54抗体
合格 DCUN1D3 DCN1样蛋白3抗体
合格 NOL12/NOP25 核仁蛋白12抗体
合格 Vasopressin 抗****/血管升压素/加压素/血管加压素抗体
合格 NOXA1 NADPH氧化酶激活蛋白1抗体
合格 NPAS2 神经细胞PAS结构域蛋白2抗体
合格 NPFF2 神经肽G蛋白偶联受体抗体
合格 NPIPL2 NPIPL2蛋白抗体
合格 NPL/N-acetylneuraminate lyase 唾液酸醛缩酶NPL抗体
合格 合格 NPRL3 氮酶调节因子3样蛋白抗体
合格 WNT9A 信号通路Wnt9a抗体
合格 NRAP 伴肌动蛋白相关锚定蛋白抗体
合格 合格 NRBP2 核受体结合蛋白2抗体
合格 NRG2 神经调节蛋白2抗体
合格 NRIP3 核受体相互作用蛋白3抗体
合格 合格 SAE1 泛素样蛋白SUMO1激活酶抗体
合格 SAMD11 SAMD11蛋白抗体
合格 Zinc Alpha 2 Glycoprotein 锌α2糖蛋白抗体
合格 Sarcalumenin 肌钙腔蛋白抗体
合格 ZNF307/ZKSCAN3 锌指蛋白307抗体
合格 PRR16/DSC54 间充质干细胞蛋白DSC54抗体
合格 Zfp91 白血病相关新基因抗体
合格 PRRC1 PRRC1蛋白抗体
合格 PRRG3 跨膜γ羧基酸蛋白3抗体
合格 PRS45 丝氨酸蛋白酶45抗体
合格 PRSS23 丝氨酸蛋白酶23抗体
合格 PRSS56 丝氨酸蛋白酶56抗体
合格 PRTG PRTG蛋白抗体
合格 WRCH1 WRCH1抗体
合格 phospho-Smad3 (Thr179) 磷酸化细胞信号转导分子SMAD3抗体