phospho-HSF1 (Ser303)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5363R
英文名称phospho-HSF1 (Ser303)
中文名称磷酸化热休克因子1抗体
别 名HSF1(phospho-Ser303); Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSTF 1; HSTF1. 说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-HSF1 (Ser303)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量57kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303:PP(p-S)PP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-HSF1 (Ser303)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008].
Function:
DNA-binding protein that specifically binds heat shockpromoter elements (HSE) and activates transcription. In highereukaryotes, HSF is unable to bind to the HSE unless the cells areheat shocked.
Subunit:
Monomer. Under normal conditions, interacts with HSP90AA1in the HSP90 multichaperone complex; the interaction preventstrimerization and activation of HSF1. On activation by heat-stressor by other factors such as metal ions, HSF1 is released from thecomplex, homotrimerizes, is hyperphosphorylated and translocated tothe nucleus where, subsequently, it can activate transcription.Binds the complex through the regulatory domain. Interacts withSYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in theHSP90 multichaperone complex; the interaction is independent of thephosphorylation state of HSF1. Interacts with MAPKAPK2.
Subcellular Location:
Cytoplasm. Nucleus. Note=Cytoplasmic duringnormal growth. On activation, translocates to nuclear stressgranules. Colocalizes with SUMO1 in nuclear stress granules.
Post-translational modifications:
Phosphorylated on multiple serine residues, a subset of whichare involved in stress-related regulation of transcriptionactivation. Constitutive phosphorylation represses transcriptionalactivity at normal temperatures. Levels increase on specificresidues heat-shock and enhance HSF1 transactivation activity.Phosphorylation on Ser-307 derepresses activation on heat-stressand in combination with Ser-303 phosphorylation appears to beinvolved in recovery after heat-stress. Phosphorylated on Ser-230by CAMK2, in vitro. Cadmium also enhances phosphorylation at thissite. Phosphorylation on Ser-303 is a prerequisite for HSF1sumoylation. Phosphorylation on Ser-121 inhibits transactivationand promotes HSP90 binding. Phosphorylation on Thr-142 alsomediates transcriptional activity induced by heat.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-induciblesumoylation occurs after 15 min of heat-shock, after which levelsdecrease and at 4 hours, levels return to control levels.Sumoylation has no effect on HSE binding nor on transcriptionalactivity. Phosphorylation on Ser-303 is a prerequisite forsumoylation.
Similarity:
Belongs to the HSF family.
Gene ID:
3297
phospho-HSF1 (Ser303)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-HSF1 (Ser303)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 MYO16 肌球蛋白16/Myosin XVI抗体
合格 PRKCBP1 蛋白激酶C结合蛋白1抗体
合格 MYOM3 肌间蛋白3抗体
合格 Rarres3 视黄酸受体应答蛋白3抗体
合格 Myosin 1C 肌球蛋白MYO1C抗体
合格 PROP1 垂体特异性同源因子抗体
合格 PROSC PROSC蛋白抗体
合格 NEK10 丝氨酸/苏氨酸蛋白激酶Nek10抗体
合格 NEK11 丝氨酸/苏氨酸蛋白激酶Nek11抗体
合格 NRK2/NEK4 丝氨酸/苏氨酸蛋白激酶2抗体
合格 NELFe 转录延伸因子E抗体
合格 合格 TLR1 Toll样受体1抗体
合格 Nesprin 2 突触核膜蛋白2抗体
合格 TOP2A DNA拓普西异构酶ⅡA抗体
合格 NFYA 核转录因子YA抗体
合格 TRF2 端粒结合蛋白2抗体
合格 NHP2L2 X连锁脑特异性BPX蛋白抗体
合格 CHRNB3 烟碱型乙酰胆碱受体β3抗体
合格 CHRNG 烟碱型乙酰胆碱受体γ抗体
合格 NIF1 锌指蛋白335抗体
合格 NIPAL4 镁转运蛋白NIPAL4抗体
合格 NISCH 新型抑癌蛋白Nischarin抗体
合格 NKAIN1 NKAIN1蛋白抗体
合格 UCP-1 线粒体脱偶连蛋白1抗体
合格 NKD2 NKD2蛋白抗体
合格 NKIRAS2 核转录因子κB抑制作用Ras样2蛋白抗体
合格 ZIK1 锌指蛋白762抗体
合格 NKPD1 NKPD1蛋白抗体
合格 合格 UCP-2 线粒体脱偶连蛋白2抗体
合格 Nkx2.4 转录因子同源蛋白NKX2.4抗体
合格 Nkx2.6 转录因子同源蛋白Nkx2.6抗体
合格 NLRP11 NLRP11蛋白抗体