Phospho-HSP27 (Ser82)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3180R
英文名称Phospho-HSP27 (Ser82)
中文名称磷酸化热休克蛋白27抗体
别 名Hsp27 (phospho S82); p-Hsp27 (phospho S82); HSP27(Phospho-Ser82);Heat shock 27kDa protein; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; Estrogen-regulated 24 kDa protein; Heat shock 25kDa protein 1; Heat shock 25kDa protein 1; Heat shock 27 kDa protein; Heat shock 27kD protein 1; Heat shock 27kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat Shock Protein 27; Heat shock protein beta 1; Heat shock protein beta-1; Heat Shock Protein27; Heat Shock Protein27; HMN2B; HS.76067; Hsp 25; Hsp 25; Hsp 27; Hsp 27; Hsp 28; Hsp 28; Hsp B1; Hsp B1; Hsp25; Hsp25; HSP27; Hsp28; Hsp28; HspB1; HspB1; HSPB1_HUMAN; SRP27; Stress responsive protein 27; Stress-responsive protein 27.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
Phospho-HSP27 (Ser82)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量27kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human HSP27 around the phosphorylation site of Ser82:QL(p-S)SG
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-HSP27 (Ser82)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Function:
Involved in stress resistance and actin organization.
Subunit:
Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1.
Subcellular Location:
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Tissue Specificity:
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Post-translational modifications:
Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress leads to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.
DISEASE:
Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
SWISS:
P04792
Gene ID:
3315
Phospho-HSP27 (Ser82)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-HSP27 (Ser82)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 LYZL2 溶菌酶样蛋白2抗体
合格 LZTR2 亮氨酸拉链样转录调节因子1抗体
合格 LZTS2 亮氨酸拉链抑癌蛋白2抗体
合格 SARDH 肌氨酸脱氢酶抗体
合格 CRLR 降钙素基因相关肽1型受体抗体
合格 MAFF 转录因子MAFF抗体
合格 MAGEA6 黑色素瘤相关抗原6抗体
合格 MAGEB10 黑色素瘤相关抗原B10抗体
合格 MAGED2 黑色素瘤相关抗原D2抗体
合格 MAGIX MAGIX蛋白抗体
合格 MAGT1 镁转运蛋白1抗体
合格 MAK16/RBM13 RNA结合蛋白MAK16抗体
合格 MAK3/C14orf35 14号染色体开放阅框35抗体
合格 MAMSTR MAMSTR蛋白抗体
合格 MAN2A2 α-甘露糖苷酶2x抗体
合格 MANBA β-甘露糖苷酶抗体
合格 MANEL MANEL蛋白抗体
合格 MAP1D 蛋氨酸氨肽酶1D抗体
合格 合格 Parkin 帕金蛋白抗体
合格 MAP1S 微管相关蛋白1S抗体
合格 合格 MAP3K13/LZK MAP3K13蛋白抗体
合格 MAPKAP3 丝裂原活化蛋白激酶活化的蛋白激酶3抗体
合格 MAPKAPK2/3 丝裂原活化蛋白激酶活化的蛋白激酶2/3抗体
合格 PD-1 程序性死亡1抗体
合格 MARS 甲硫氨酸转运RNA合成酶抗体
合格 MARS2 甲硫氨酸转运RNA合成酶2抗体
合格 MARVELD3 MARVEL蛋白家族D3抗体
合格 MBOAT1 溶血磷脂酰酰基转移酶1抗体
合格 phospho-MCAK (Ser95) 磷酸化有丝分裂着丝粒相关驱动蛋白抗体
合格 MCART1 MCART1蛋白抗体
合格 phospho-MCL1 (Ser159) 磷酸化髓样细胞白血病1抗体
合格 合格 MCRS1/p78 细胞周期调控因子P78抗体
合格 phospho-MCSF Receptor (Tyr561) 磷酸化巨噬细胞集落刺激因子受体抗体