phospho-IGF1R (Tyr1280)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5448R
英文名称phospho-IGF1R (Tyr1280)
中文名称磷酸化胰岛素样生长因子1受体抗体
别 名IGF1R (phospho-Tyr1280); IGF1R (phospho Y1280); IGF1R (phospho Tyr1280); p-IGF1R (Tyr1280); p-IGF1R (Y1280); CD221; CD221 antigen; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor 1 receptor beta chain; IGF 1 receptor; IGF 1R; IGF I receptor; IGF1R; IGFIR; IGFIRC; Insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor precursor; JTK13; MGC142170; MGC142172; IGF1 Receptor; IGF1R_HUMAN; IGF-IR.
说 明 书100ul
产品类型磷酸化抗体
研究领域信号转导 生长因子和** 转录调节因子 激酶和磷酸酶 细胞膜受体 糖尿病
抗体来源Rabbit
克隆类型Polyclonal
phospho-IGF1R (Tyr1280)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量69/150kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr1280:SF(p-Y)YS
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-IGF1R (Tyr1280)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008].
Function:
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
Subunit:
Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Tissue Specificity:
Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
Post-translational modifications:
The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
DISEASE:
Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain.
SWISS:
P08069
Gene ID:
3480
phospho-IGF1R (Tyr1280)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-IGF1R (Tyr1280)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 FDC-SP 滤泡树突细胞分泌蛋白抗体
合格 SPINK4 胃肠肽SPINK4抗体
合格 SPINK6 丝氨酸蛋白酶抑制剂SPINK6抗体
合格 SPINK8 丝氨酸蛋白酶抑制剂SPINK8抗体
合格 phospho-splicing factor 1 (Ser82) 磷酸化剪接因子1抗体
合格 SPOCK3 富含半胱氨酸酸性分泌/骨粘连蛋白多糖抗体
合格 MMP6/MPHOSPH6 基质金属蛋白酶6抗体
合格 MMRN2 细胞外基质糖蛋白MMRN2抗体
合格 phospho-MNK1 (Thr385) 磷酸化MAP激酶相互作用丝氨酸/苏氨酸激酶1抗体
合格 MNK2 MAP激酶相互作用丝氨酸/苏氨酸激酶2抗体
合格 MOB4A MOB4A蛋白抗体
合格 MOG1 Ran结合蛋白MOG1抗体
合格 MOGAT2 MOGAT2蛋白抗体
合格 HSPB4/Alpha A Crystallin 热休克蛋白β4抗体
合格 MON1A MON1A蛋白抗体
合格 合格 MON1B/HSRG1 HSV-1刺激相关蛋白抗体
合格 MORN4/C10orf83 10号染色体开放阅读框83抗体
合格 MOSC2 MOSC2蛋白抗体
合格 Cytokeratin 15 细胞角蛋白15抗体
合格 MOV10L1 莫洛尼白血病病毒10样蛋白1抗体
合格 MOXD1 单加氧酶X抗体
合格 MPPED1 金属磷酸酯酶1抗体
合格 MRAP 脂肪组织特异性低分子量蛋白抗体
合格 phospho-MRCL3 (Thr18 + Ser19) 磷酸化肌球蛋白调节轻链MRLC3抗体
合格 phospho-Mre11 (Ser264) 磷酸化DNA损伤关键蛋白Mre11抗体
合格 MRFAP1L1 T**细胞活化蛋白样蛋白MRFAP1L1抗体
合格 合格 MRGPRA 腺嘌呤受体抗体
合格 HFH4 叉头蛋白J1抗体
合格 MRPL13 线粒体核糖体蛋白L13抗体
合格 CCKBR 促胃泌素受体抗体
合格 MRPL32 线粒体核糖体蛋白L32抗体