phospho-Integrin beta 1(Tyr783)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 16635R
英文名称phospho-Integrin beta 1(Tyr783)
中文名称磷酸化整合素β1/Integrin β1抗体
别 名Integrin beta 1 (phospho Y783); p-Integrin beta 1 (phospho Y783); beta 1 subunit; CD 29; CD29; CD29 antigen; Fibrinogen Receptor beta subunit; Fibronectin receptor subunit beta; FNRB; Glycoprotein IIa; GP IIa; GPIIA; integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12); Integrin beta-1; Integrin VLA 4 subunit beta; integrin VLA-4 beta subunit; Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITB1_HUMAN; ITGB 1; ITGB1; MDF2; MSK12; OTTHUMP00000019420; Very Late Activation Protein beta Polypeptide; Very late activation protein, beta polypeptide; VLA BETA; VLA-4 subunit beta; VLA-BETA; VLAB; VLAbeta.
说 明 书100ul
产品类型磷酸化抗体
研究领域细胞生物 神经生物学 信号转导 干细胞
抗体来源Rabbit
克隆类型Polyclonal
phospho-Integrin beta 1(Tyr783)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量86kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human Integrin beta 1 around the phosphorylation site of Tyr783:NPI(p-Y)KS
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Integrin beta 1(Tyr783)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis.
Subcellular Location:
Cell membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection > lamellipodium. Cell projection > ruffle. Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner.
Tissue Specificity:
Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).
Post-translational modifications:
The cysteine residues are involved in intrachain disulfide bonds.
Similarity:
Belongs to the integrin beta chain family.
Contains 1 VWFA domain.
Gene ID:
3688
phospho-Integrin beta 1(Tyr783)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Integrin beta 1(Tyr783)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 C2orf72 2号染色体开放阅读框72抗体
合格 C2orf88 2号染色体开放阅读框88抗体
合格 C3IP1 补体C3IP1抗体
合格 C3orf14 3号染色体开放阅读框14抗体
合格 C3orf25 3号染色体开放阅读框25抗体
合格 C3orf38 3号染色体开放阅读框38抗体
合格 C3orf58 3号染色体开放阅读框58抗体
合格 C4orf29 4号染色体开放阅读框29抗体
合格 C4orf52 4号染色体开放阅读框52抗体
合格 E cadherin 上皮钙粘附分子抗体
合格 C5orf22 5号染色体开放阅读框22抗体
合格 C5orf24 5号染色体开放阅读框24抗体
合格 C5orf34 5号染色体开放阅读框34抗体
合格 C5ORF42 5号染色体开放阅读框42抗体
合格 C5orf48 5号染色体开放阅读框48抗体
合格 C5orf49 5号染色体开放阅读框49抗体
合格 合格 CD7 CD7抗体
合格 C5orf54 5号染色体开放阅读框54抗体
合格 C5orf55 5号染色体开放阅读框55抗体
合格 C6orf132 6号染色体开放阅读框132抗体
合格 C6orf136 6号染色体开放阅读框136抗体
合格 C6orf145 6号染色体开放阅读框145抗体
合格 C6ORF173 6号染色体开放阅读框173抗体
合格 C6orf195 6号染色体开放阅读框195抗体
合格 C6orf201 6号染色体开放阅读框201抗体
合格 C6orf203 6号染色体开放阅读框203抗体
合格 C6orf204 6号染色体开放阅读框204抗体
合格 C6orf206 6号染色体开放阅读框206抗体
合格 合格 CD63 黑色素瘤相关抗原抗体
合格 C6orf222 6号染色体开放阅读框222抗体
合格 C6orf225 6号染色体开放阅读框225抗体
合格 C6orf50 6号染色体开放阅读框50抗体
合格 GlyR alpha 1 + 2 + 3 甘氨酸受体alpha 1/2/3型抗体