Phospho-IRAK1 (Thr387)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3194R
英文名称Phospho-IRAK1 (Thr387)
中文名称磷酸化白介素-1受体相关激酶1抗体
别 名IRAK (phospho T387); p-IRAK (phospho T387); IRAK1 (Phospho-Thr387); IRAK1 (Phospho-T387); IRAK1 (p-Thr387); Il1rak; Il1rak; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 2; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; IRAK1; IRAK1; IRAK1_HUMAN; IRAK2; IRAK2; mPLK; mPLK; Pelle; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 信号转导 细胞凋亡
Phospho-IRAK1 (Thr387)抗体抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量78kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr387 [RG(p-T)LA]:RG(p-T)LA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-IRAK1 (Thr387)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3
Subunit:
Homodimer. Interacts with TOLLIP; this interaction occurs in the cytosol prior to receptor activation. Interacts with MYD88; this interaction recruits IRAK1 to the stimulated receptor complex. Interacts with IL1RL1. Interacts with IRAK1BP1. Associates with TRAF6, PELI1 and IRAK4; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Interacts (when polyubiquitinated) with IKBKG/NEMO.
Subcellular Location:
Cytoplasm. Nucleus. Note=Translocates to the nucleus when sumoylated.
Tissue Specificity:
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
Post-translational modifications:
Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity.
Polyubiquitinated after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation.
Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain.
SWISS:
P51617
Gene ID:
3654
Phospho-IRAK1 (Thr387)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-IRAK1 (Thr387)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 FAM116A FAM116A蛋白抗体
合格 合格 合格 GRP75 G蛋白偶联受体75抗体
合格 FAM118A FAM118A蛋白抗体
合格 FAM118B FAM118B蛋白抗体
合格 FAM119A FAM119A蛋白抗体
合格 合格 FAM124A FAM124A蛋白抗体
合格 DDX19A ATP依赖解旋酶DDX19A抗体
合格 FAM131B FAM131B蛋白抗体
合格 FAM131A FAM131A蛋白抗体
合格 FAM132A 脂肪源性胰岛素增敏因子抗体
合格 FAM135A FAM135A蛋白抗体
合格 FAM136a FAM136a蛋白抗体
合格 合格 FAM149B1 FAM149B1蛋白抗体
合格 合格 FAM151B FAM151B蛋白抗体
合格 FAM154B FAM154B蛋白抗体
合格 FAM160B1 FAM160B1蛋白抗体
合格 FAM161B FAM161B蛋白抗体
合格 FAM167A FAM167A蛋白抗体
合格 CCL28 粘膜相关上皮趋化因子28抗体
合格 FAM171A1 FAM171A1蛋白抗体
合格 FAM171A2 FAM171A2蛋白抗体
合格 Gremlin 骨形态形成蛋白拮抗蛋白抗体
合格 FAM189A1 FAM189A1蛋白抗体
合格 合格 P4HB 蛋白质二硫键异构酶抗体
合格 FAM190B FAM190B蛋白抗体
合格 FAM194A FAM194A蛋白抗体
合格 FAM194B FAM194B蛋白抗体
合格 FAM196B FAM196B蛋白抗体
合格 FAM199X FAM199X蛋白抗体
合格 合格 合格 PAFR/PAF Receptor 血小板活化因子受体抗体
合格 CD80 刺激分子B7-1蛋白抗体
合格 Rho C RhoC抗体
合格 MTHFR 亚甲基四氢叶酸还原酶MTHFR抗体
合格 FAM200A FAM200A蛋白抗体