phospho-JNK1 (Thr183)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 17591R
英文名称phospho-JNK1 (Thr183)
中文名称磷酸化氨基末端激酶1抗体
别 名JNK1 (phospho T183); p-JNK1 (phospho T183); AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK 1; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; mapk8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08_HUMAN; p54 gamma; Prkm8; Protein kinase JNK1; Protein kinase, mitogen-activated, 8; SAPK 1; SAPK gamma; SAPK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-JNK1 (Thr183)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Zebrafish, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量50kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183:MM(p-T)PY
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-JNK1 (Thr183)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]
Function:
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Post-translational modifications:
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
Similarity:
Belongs to the protein kinase superfamily.
CMGC Ser/Thr protein kinase family.
MAP kinase subfamily.
Contains 1 protein kinase domain.
Gene ID:
5599
phospho-JNK1 (Thr183)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-JNK1 (Thr183)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 ENPP5 磷酸酶/磷酸二酯酶家族成员5抗体
合格 ENT2 核苷转运蛋白ENT2抗体
合格 phospho-EphB1 (Tyr928) 磷酸化酪氨酸蛋白激酶受体B1抗体
合格 phospho-EPO Receptor (Tyr485) 磷酸化红细胞生成素受体抗体
合格 Phospho-HER3 (Tyr1276) 磷酸化HER3抗体
合格 ERF 转录因子ERF抗体
合格 合格 Syndecan-1 多配体蛋白聚糖1抗体
合格 Epsin 1 内吞作用辅助蛋白EPN1抗体
合格 Epsin 2 内吞作用辅助蛋白EPN2抗体
合格 Integrin Alpha V + Beta 3 (CD51+CD61) 整合素αVβ3抗体
合格 ETL EGF**受体7跨膜结构域蛋白1抗体
合格 ETV3L ETV3L蛋白抗体
合格 EXOC2 胞吐囊复合体蛋白2抗体
合格 Exonuclease 1 核酸外切酶1抗体
合格 EXOSC10 多发性肌炎/硬皮病自身抗原2抗体
合格 EXOSC7 核糖体RNA合成蛋白42抗体
合格 Factor I light chain 补体因子I轻链抗体
合格 FAM134B FAM134B蛋白抗体
合格 phospho-FANCA (Ser1149) 磷酸化范可尼贫血组蛋白A抗体
合格 phospho-FANCD2(Ser1404) 磷酸化FANCD2抗体
合格 TRRAP 转录因子相关蛋白TRRAP抗体
合格 Oxytocin R 催产素受体(缩宫素受体)抗体
合格 合格 FCGRT IgG-Fc片断受体转运蛋白α抗体
合格 FHOD3 FHOD3蛋白抗体
合格 Nogo A+B 轴索过度生长抑制因子A+B抗体 Nogo-B/A
合格 Ficolin 2 弹性蛋白结合蛋白Fcn2抗体
合格 FBX09 F-box蛋白9抗体
合格 FENS1 磷酸肌醇结合蛋白1抗体
合格 PDGFBB 血小板源性生长因子BB抗体
合格 FIZ1 锌指蛋白798抗体
合格 FKBP10 肽基脯氨酰顺反异构酶FKBP10抗体
合格 STAT1 信号转导与转录激活因子1抗体
合格 FLNC 细丝蛋白2抗体
合格 phospho-FLNC (2233) 磷酸化细丝蛋白2抗体