phospho-MAP3K9+MAP3K10(Thr312 + Thr266)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 6779R
英文名称phospho-MAP3K9+MAP3K10(Thr312 + Thr266)
中文名称磷酸化丝裂原活化蛋白激酶3K9抗体
别 名MAP3K9 +MAP3K10 (phospho T312 + T266); MAP3K9 + MAP3K10(phospho T312 + T266); phospho-MAP3K9(Thr312)/MAP3K10( Thr266); p-MAP3K9; M3K9_HUMAN; M3K10_HUMAN; MAPKKK9; MEKK10; MEKK9; Mitogen activated protein kinase kinase kinase 10; Mitogen activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 2; MKN28 derived nonreceptor_type serine/threonine kinase; MKN28 kinase; MLK1; MLK2; MST; PRKE1; Protein kinase MST.
说 明 书100ul
产品类型磷酸化抗体
研究领域细胞生物 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-MAP3K9+MAP3K10(Thr312 + Thr266)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量121/105kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human MAP3K9/MAP3K10 around the phosphorylation site of Thr312/Thr266:AG(p-T)YA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-MAP3K9+MAP3K10(Thr312 + Thr266)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis. [provided by RefSeq, Jul 2008].
Function:
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
Subunit:
Homodimer.
Tissue Specificity:
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Post-translational modifications:
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308.
DISEASE:
May play a role in esophageal cancer susceptibility and/or development.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
SWISS:
P80192
Gene ID:
4293
phospho-MAP3K9+MAP3K10(Thr312 + Thr266)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-MAP3K9+MAP3K10(Thr312 + Thr266)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 FAM71A FAM71A蛋白抗体
合格 合格 FAM83F FAM83F蛋白抗体
合格 合格 CD36 CD36抗体
合格 FAM98A FAM98A蛋白抗体
合格 ILDR1 **球蛋白样结构域受体1抗体
合格 合格 ILVBL 乙酰乳酸合成酶蛋白抗体
合格 GFAP delta 胶质纤维酸性蛋白GFAPδ抗体
合格 CD276 CD276抗体
合格 Gigaxonin 巨轴索神经病蛋白GAN抗体
合格 合格 Kinesin/KIF5B 驱动蛋白KNS1抗体
合格 RP1 视网膜色素变性蛋白1抗体
合格 Gliomedin 神经胶质蛋白(肝癌相关基因2)抗体
合格 PD-L1 程序性死亡配体1抗体
合格 C1QL1 补体组分1q子成分样蛋白1抗体
合格 Elastin 项韧带弹性蛋白抗体
合格 Caspase-12 半胱胺酸蛋白酶蛋白-12抗体
合格 procollagen type IIA Ⅱ型胶原α1 N端前体肽抗体
合格 CDH18 钙粘附分子18抗体
合格 CNTN4/AXCAM 轴突相关粘附分子抗体
合格 PTHLH 甲状旁腺**相关蛋白抗体
合格 合格 JAMC 连接粘附分子C抗体
合格 Myelin PLP 髓磷酯髓鞘蛋白1抗体
合格 NCAM2 神经细胞粘附分子2抗体
合格 NLGN2 神经连接蛋白2抗体
合格 PCDH12 原钙粘附蛋白12/血管内皮钙粘蛋白2抗体