Phospho-MAPKAPK2 (Thr222)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3261R
英文名称Phospho-MAPKAPK2 (Thr222)
中文名称磷酸化丝裂原活化蛋白激酶活化的蛋白激酶2抗体
别 名MAPKAP Kinase 2 (phospho S222); p-MAPKAP Kinase 2 (phospho S222); 3PK; AA960234; MAP kinase activated protein kinase 2; MAP kinase activated protein kinase 3; MAPK activated protein kinase 2; MAPK activated protein kinase 3; MAPKAP kinase 2; MAPKAP kinase 3; MK2; MK3; MAPK2_HUMAN; OTTHUMP00000034531; Rps6kc1.
JournalPMIDIFApplicationOsteoarthritis and Cartilage (2012)229357884.2620WB
说 明 书100ul
产品类型磷酸化抗体
研究领域**学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
Phospho-MAPKAPK2 (Thr222)抗体交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, Monkey,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量46kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Thr222:LT(p-T)PC
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-MAPKAPK2 (Thr222)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
MAP kinase activated protein kinase 2 (MAPKAP Kinase 2), also known as p45 hsp27 kinase, is a 45-54 kDa serine/threonine protein kinase that contains a proline rich sequence and two putative SH3 binding sites. MAPKAP Kinase 2 is activated in response to stress, IL1 and TNF, possibly catalyzed by p38/Hog dependent phosphorylation. One of the major substrates of MAPKAP Kinase 2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. MAPKAP2 is implicated in several disorders including ischemic brain injury and heart failure and has been shown to be important in regulating stress resistance and the production of TNF alpha.
Function:
Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Subunit:
Heterodimer with p38-alpha/MAPK14. The heterodimer with p38-alpha/MAPK14 forms a stable complex: molecules are positioned 'face to face' so that the ATP-binding sites of both kinases are at the heterodimer interface. Interacts with PHC2.
Subcellular Location:
Cytoplasm. Nucleus. Note=Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm.
Tissue Specificity:
Expressed in all tissues examined.
Post-translational modifications:
Sumoylation inhibits the protein kinase activity.
Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.
Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
SWISS:
P49137
Gene ID:
9261
Phospho-MAPKAPK2 (Thr222)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-MAPKAPK2 (Thr222)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
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合格 合格 TNNI3 心肌肌钙蛋白I抗体
合格 Myeloperoxidase 髓过氧化物酶抗中性粒细胞胞质IgG抗体
合格 ADRA2 ADRA2肾上腺素能受体2抗体
合格 beta 3 Adrenergic Receptor β3肾上腺素能受体抗体
合格 ABCD2 三磷酸腺苷结合盒转运蛋白2抗体
合格 ROR gamma T 维甲酸相关孤儿受体γt抗体
合格 合格 Cardiac Troponin T 心肌特异性肌钙蛋白T抗体
合格 合格 KLF4 肠道内富含的Kruppel样因子/上皮锌指蛋白4抗体
合格 合格 PI3 Kinase p110 delta 磷脂酰肌醇激酶催化亚单位D抗体
合格 合格 Growth hormone receptor 生长**受体抗体
合格 合格 CD105 内皮糖蛋白抗体
合格 phospho-AMPK alpha-1(Ser184) 磷酸化腺苷单磷酸活化蛋白激酶α1抗体
合格 Brachyury Brachyury蛋白抗体
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合格 ALDH1A2 乙醛脱氢酶2型抗体
合格 细胞表面趋化因子受体8抗体
合格 NF-H 高分子量神经丝蛋白抗体
合格 NMDAR1 离子型谷氨酸受体1抗体
合格 BMP2 骨形态发生蛋白2抗体
合格 合格 合格 CD8 CD8抗体
合格 HS6ST1 硫酸乙酰肝素6脑苷脂转硫酸酶1抗体
合格 NELL1 蛋白激酶C结合蛋白NELL1抗体
合格 I-309/CCL1 嗜酸粒细胞趋化蛋白CCL1抗体
合格 AKT2 蛋白激酶B2抗体
合格 CD101 CD101抗体