Phospho-MLK3(Thr277 + Ser281)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3280R
英文名称Phospho-MLK3(Thr277 + Ser281)
中文名称磷酸化丝氨酸/苏氨酸蛋白激酶MLK3抗体
别 名MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1; PTK1; RHOE; SH3 domain containing proline rich kinase; SPRK; Src-homology 3 domain-containing proline-rich kinase; 2610017K16Rik; EC 2.7.11.25; M3K11_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域**学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
Phospho-MLK3(Thr277 + Ser281)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Guinea Pig,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量93kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human MLK3 around the phosphorylation site of Thr277/Ser281:K(p-T)TQM(p-S)AA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-MLK3(Thr277 + Ser281)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Members of the mixed-lineage kinase (MLK) family (including MLK1, MLK2, MLK3, and dual leucine zipper kinase [DLK]) are serine/threonine protein kinases that are expressed in multiple cell types. MLK3 is activated by phosphorylation in response to stress stimuli (e.g., inflammatory responses, UV, chemical stress) that are coupled to the small GTPase, Cdc42/rac. MLK3 is a multifunctional kinase that plays an essential role in several signaling pathways, including mitogen-activated protein kinase (i.e. activation of JNK and p38), IkappaB/NFkappaB, and p70 S6 kinase. Indeed MLK3 signaling occurs through multiple signaling domains in this protein kinase including (from N- to C-terminal) a glycine-rich domain, Src homology 3 (SH3) domain, a kinase domain, a zipper domain, a Cdc42/rac interactive binding (CRIB) domain and a Pro/Ser/Thr-rich domain. Phosphorylation of MLK3 occurs on multiple residues including threonine 277 and serine 281 within the activation loop of the kinase domain.
Function:
Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
Subunit:
Homodimer; undergoes dimerization during activation.
Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Note=Location is cell cycle dependent.
Tissue Specificity:
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and ***** lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
Post-translational modifications:
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
SWISS:
Q16584
Gene ID:
4296
Phospho-MLK3(Thr277 + Ser281)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-MLK3(Thr277 + Ser281)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 C1ORF74 1号染色体开放阅读框74抗体
合格 C1orf94 1号染色体开放阅读框94抗体
合格 C1orf41 1号染色体开放阅读框41抗体
合格 C1orf57 1号染色体开放阅读框57抗体
合格 ECRG4 食道癌相关基因4蛋白抗体
合格 C2orf60 2号染色体开放阅读框60抗体
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合格 C2orf42 2号染色体开放阅读框42抗体
合格 C2orf55 2号染色体开放阅读框55抗体
合格 C2orf57 2号染色体开放阅读框57抗体
合格 C2orf89 2号染色体开放阅读框89抗体
合格 合格 C2orf29 2号染色体开放阅读框29抗体
合格 合格 C3orf17 3号染色体开放阅读框17抗体
合格 C3orf23 3号染色体开放阅读框23抗体
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合格 CSEN 钾通道相互作用蛋白3抗体
合格 CCAP 心动加速蛋白多肽抗体
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合格 Titin/CMD1G 扩张型心肌病蛋白1G抗体
合格 MYH7B 肌球蛋白重链7抗体
合格 WIPF2 WASP结合蛋白抗体
合格 Thymosin beta 4 胸腺素β4抗体
合格 Rap2A Rap2A抗体
合格 TFR2 转铁蛋白受体2抗体
合格 C11ORF46 11号染色体开放阅读框46抗体
合格 C11ORF77 11号染色体开放阅读框77抗体
合格 合格 C11ORF16 11号染色体开放阅读框16抗体
合格 C11orf74 11号染色体开放阅读框74抗体