phospho-MRCL3 (Thr18 + Ser19)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 17750R
英文名称phospho-MRCL3 (Thr18 + Ser19)
中文名称磷酸化肌球蛋白调节轻链MRLC3抗体
别 名MRCL3 (phospho T18 + S19); ML12A_HUMAN; MLC 2B; MLC-2B; MLCB; MYL12A; Myosin regulatory light chain 12A; Myosin regulatory light chain 2; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain MRCL3; Myosin regulatory light chain MRLC3; Myosin RLC; Myosin, light polypeptide, regulatory, non sarcomeric (20kD); nonsarcomeric; RLC.
说 明 书100ul 200ul
研究领域肿瘤 心血管 细胞生物 信号转导 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
phospho-MRCL3 (Thr18 + Ser19)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Zebrafish, Sheep,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量20kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human MRCL3 around the phosphorylation site of Thr18 + Ser19:RA(p-T)(p-S)N
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-MRCL3 (Thr18 + Ser19)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Myosin regulatory light chains, including MRCL3 (also known as MRLC3 or MLCB), MRLC2 (also known as MLC-B) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined.
Function:
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Post-translational modifications:
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Similarity:
Contains 3 EF-hand domains.
Gene ID:
10627
phospho-MRCL3 (Thr18 + Ser19)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-MRCL3 (Thr18 + Ser19)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 C16orf48 16号染色体开放阅读框48抗体
合格 PDZD9 PDZ结构域PDZK9蛋白抗体
合格 SLC39A11 溶质载体家族39成员11抗体
合格 XTP4 乙型肝炎病毒X蛋白反转录蛋白4抗体
合格 C17orf42 17号染色体开放阅读框42抗体
合格 合格 C17orf57 17号染色体开放阅读框57抗体
合格 C17orf74 17号染色体开放阅读框74抗体
合格 C17orf75 17号染色体开放阅读框75抗体
合格 C17orf77 17号染色体开放阅读框77抗体
合格 C17orf78 17号染色体开放阅读框78抗体
合格 C17orf82 17号染色体开放阅读框82抗体
合格 合格 C17orf97 17号染色体开放阅读框97抗体
合格 合格 pan 14-3-3 14-3-3蛋白抗体
合格 RIMKLA 核糖体蛋白S6修饰样蛋白A抗体
合格 FAM76B FAM76B蛋白抗体
合格 ENAH ENAH蛋白抗体
合格 EGFL8 表皮生长因子样蛋白8抗体
合格 C8orf70 8号染色体开放阅读框70抗体
合格 合格 LAS2 肺腺瘤易感蛋白2抗体
合格 C19orf21 19号染色体开放阅读框21抗体
合格 C19orf46 突触核膜蛋白4抗体
合格 C19orf47 19号染色体开放阅读框47抗体
合格 C19orf54 19号染色体开放阅读框54抗体
合格 VGLUT2 囊泡谷氨酸转运蛋白2抗体