phospho-Nrf2 (Ser40)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 2013R
英文名称phospho-Nrf2 (Ser40)
中文名称磷酸化核因子2相关因子2(Ser40)抗体
别 名Nrf2 (phospho S40); p-Nrf2 (phospho S40); Nuclear factor-E2 related factor2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN.
JournalPMIDIFApplicationAntioxidants & redox signaling (2013)238757767.1890N/AFree Radical Biology and Medicine (2015)261630015.7360ICCFree Radical Biology and Medicine (2013)234347665.2710WB/IHC-frFree Radical Biology and Medicine (2012)230224085.2710WBTranslational Research (2016)267468025.0300WBInfection and Immunity (2016)271857863.7100IF(ICC)PLoS One (2014)254195703.3700IHC-POxidative Medicine and Cellular Longevity (N/A)246692843.3630IHC-PBehavioural Brain Research (2016)266984003.0280WBFood and Chemical Toxicology (2013)240361423.0100WbFood and Chemical Toxicology (2013)240361423.0100WBJournal of Agricultural and Food Chemistry (2013)237312452.9060 IHC Journal of Neural Transmission (2014) 25504008 2.8710 WB Journal of Functional Foods (2013) Others 2.7420 WB Molecules (2015) 25671364 2.0950 IHC-P
说 明 书100ul
产品类型磷酸化抗体
研究领域**学 染色质和核信号 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-Nrf2 (Ser40)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量68kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 [DF(p-S)QR]:DF(p-S)QR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Nrf2 (Ser40)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP) subfamily of basic /leucine zipper (bZIP) transcription factors.
Function:
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Subunit:
Heterodimer. Forms a ternary complex with PGAM5 and KEAP1. May bind DNA with an unknown protein. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1.
Subcellular Location:
Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Tissue Specificity:
Widely expressed. Highest expression in ***** muscle, kidney, lung, liver and in fetal muscle.
Post-translational modifications:
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus (By similarity).
Similarity:
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain.
SWISS:
Q16236
Gene ID:
4780
phospho-Nrf2 (Ser40)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Nrf2 (Ser40)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-SHP2 (Tyr81) 磷酸化蛋白酪氨酸磷酸酶2抗体
合格 phospho-SOX9(Ser181) 磷酸化转录因子SOX9蛋白抗体
合格 phospho-c-Raf(Ser339) 磷酸化原癌基因c-Raf抗体
合格 phospho-RAD9(Tyr28) 磷酸化细胞周期检查控制蛋白质抗体
合格 phospho-NFKB p65(Thr435) 磷酸化细胞核因子抗体
合格 phospho-NFKB p65(Thr254) 磷酸化细胞核因子抗体
合格 phospho-NFKB p65(Thr505) 磷酸化细胞核因子抗体
合格 phospho-RPS6KB1(Ser417) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-RPS6KB1(Ser427) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-RPS6KB1(Ser441+Thr444) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-ROCK2(Tyr256) 磷酸化Rho相关蛋白激酶2抗体
合格 phospho-RAD51(Thr309) 磷酸化Rad51抗体
合格 phospho-RPS6KA1(Ser352) 磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体
合格 phospho-p107(Ser975) 磷酸化视网膜母细胞瘤样蛋白p107抗体
合格 phospho-STAT5b (Ser731) 磷酸化信号转导和转录激活因子5b抗体
合格 LZTS1 假定肿瘤抑制亮氨酸拉链蛋白1抗体
合格 Ensconsin 上皮细胞微管相关蛋白7抗体
合格 MBNL3 毒蕈碱样蛋白3抗体
合格 合格 APRIL/TNFSF13 肿瘤坏死因子配体超家族成员13抗体
合格 LGALS3BP 可溶性半乳糖凝集素3结合蛋白抗体
合格 MARK2 丝氨酸/苏氨酸蛋白激酶MARK2抗体