phospho-p107(Ser975)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5696R
英文名称phospho-p107(Ser975)
中文名称磷酸化视网膜母细胞瘤样蛋白p107抗体
别 名RBL1(phospho S975); Cellular protein 107; CP107; PRB1; RBL1; Retinoblastoma like protein 1; RBL1_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 信号转导 干细胞 细胞周期蛋白 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
phospho-p107(Ser975)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量121kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human RBL1 around the phosphorylation site of Ser975:PG(P-S)PR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-p107(Ser975)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The pocket protein family consists of three structurally and functionally related proteins, Rb (retinoblastoma), p107, and p130. This family of tumor suppressors function to regulate important cellular transcription factors, such as the E2F family. The E2F proteins regulate the expression of genes whose products are important for cell cycle progression. The inactivation Rb is catalyzed by CDK phosphorylation thereby releasing E2F during the G1-S phase cellular progression. Unchecked inactivation of Rb in G1 phase has been indicated as a universal mechanism underlying cellular transformation . While Rb has been the most studied among the pocket proteins, p107 and p130 have also been shown to be key regulators of E2F. Several studies have also provided evidence that p107/p130 provide different functions in E2F regulation than does Rb. Rb, p107, and p130 each contain a conserved 'A/B pocket', which is the target of several viral oncoproteins, namely SV40 large T-antigen and adenovirus E1A. There are two isoforms.
Function:
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
Subunit:
Interacts with SUV420H1, SUV420H2 and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with SV40 and JC virus large T antigens.
Subcellular Location:
Nucleus.
Post-translational modifications:
Exists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
Similarity:
Belongs to the retinoblastoma protein (RB) family.
SWISS:
P28749
Gene ID:
5933
phospho-p107(Ser975)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-p107(Ser975)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-TrkB (Tyr705) 磷酸化酪氨酸激酶B抗体
合格 合格 FXR1 脆性X相关蛋白1抗体
合格 phospho-Kv2.1(Tyr128) 磷酸化钾通道蛋白DRK1抗体
合格 Phospho-PDPK1(Ser393) 磷酸化3磷酸肌醇依赖性蛋白激酶1抗体
合格 phospho-PIK3R1(Tyr368) 磷酸化磷脂酰肌醇激酶抗体
合格 Phospho-Paxillin(Tyr88) 磷酸化桩蛋白Paxillin抗体
合格 phospho-AMPK alpha-1 (Thr183) 磷酸化腺苷单磷酸活化蛋白激酶α1抗体
合格 phospho-PTPN7 (Ser246) 磷酸化非受体型蛋白酪氨酸磷酸酶7抗体
合格 合格 phospho-PKC delta (Ser645) 磷酸化蛋白激酶C亚性D型抗体
合格 phospho-PRKD3(Ser41) 磷酸化蛋白激酶C nu型抗体
合格 phospho-PTPN6(Tyr564) 磷酸化蛋白酪氨酸磷酸酶1C抗体
合格 phospho-PTPN6(Ser591) 磷酸化蛋白酪氨酸磷酸酶1C抗体
合格 phospho-PIK3R2 (Tyr467) 磷酸化磷脂酰肌醇激酶p85β抗体
合格 phospho-PRKCQ (Ser695) 磷酸化蛋白激酶C theta抗体
合格 合格 phospho-14-3-3 epsilon (Thr232) 磷酸化14-3-3E蛋白抗体
合格 合格 phospho-P53(Thr55) 磷酸化肿瘤抑制基因P53抗体
合格 phospho-TNIK (Ser764) 磷酸化TRAF2和NCK激酶相互作用蛋白抗体
合格 phospho-TERT(Ser1125) 磷酸化端粒酶逆转录酶抗体
合格 Phospho-Tuberin(Ser1418) 磷酸化结节性硬化蛋白抗体
合格 Phospho-Smad3 (Ser425) 磷酸化细胞信号转导分子SMAD3抗体
合格 phospho-Alpha synuclein (Tyr136) 磷酸化核突触蛋白α抗体
合格 phospho-Alpha synuclein(Ser129) 磷酸化核突触蛋白α抗体
合格 phospho-SYN1(Ser62+Ser67) 磷酸化神经突触素1抗体