phospho-PPAR alpha (Ser12)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 4055R
英文名称phospho-PPAR alpha (Ser12)
中文名称磷酸化α型-过氧化酶活化增生受体抗体
别 名PPAR alpha (phospho-Ser12); PPAR alpha (phospho-S12); p-PPAR alpha (Ser12); p-PPAR alpha (S12); hPPAR; MGC2237; MGC2452; NR1C1; Nuclear receptor subfamily 1 group C member 1; Peroxisome Proliferator Activated Receptor alpha; PPAR; PPAR alpha; PPARA; OTTHUMP00000197740; OTTHUMP00000197741; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; PPARA_HUMAN; PPARalpha.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-PPAR alpha (Ser12)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量52kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12:PL(p-S)PL
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-PPAR alpha (Ser12)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Function:
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
Subunit:
Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with AKAP13, LPIN1 and PRDM16. Also interacts with PPARBP coactivator in vitro. Interacts with CITED2; the interaction stimulates its transcriptional activity (By similarity). Interacts with NCOA3 and NCOA6 coactivators. Interacts with ASXL1 AND ASXL2.
Subcellular Location:
Nucleus.
Tissue Specificity:
Skeletal muscle, liver, heart and kidney.
Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
SWISS:
Q07869
Gene ID:
5465
phospho-PPAR alpha (Ser12)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-PPAR alpha (Ser12)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 CD68 CD68抗体
合格 PIWIL4 piwi样4蛋白抗体
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合格 TGF-beta 3 转移生长因子β3抗体（TGFβ3）
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合格 LC3C 微管相关蛋白轻链3C抗体
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合格 VWF 血管假性血友病因子/血管性血友病因子抗体
合格 CD33 CD33抗体
合格 Semaphorin 5A 跨膜蛋白SEMA5A抗体
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合格 合格 Tau 微管相关蛋白抗体
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合格 合格 LOX 1 凝集素样氧化型低密度脂蛋白受体抗体
合格 GLRB 甘氨酸受体β/GlyR β抗体
合格 Dlx5 同源转录因子DLX5抗体
合格 SLC27A4 长链脂肪酸转运蛋白4抗体
合格 DAL1/ EPB41L3 细胞骨架4.1蛋白家族DAL1抗体
合格 CCL27 皮肤T细胞虏获趋化因子抗体
合格 IRX1 Iroquois同源蛋白1抗体
合格 Estrogen Related Receptor beta 雌**相关受体β抗体
合格 合格 BTN2A1 嗜乳脂蛋白2亚型A1抗体