Phospho-PPAR Gamma (ser273)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 4888R
英文名称Phospho-PPAR Gamma (ser273)
中文名称磷酸化过氧化酶活化增生受体γ抗体 PPARγ
别 名Phospho-PPAR Gamma(ser273); P-PPAR Gamma (Phospho-ser273); CIMT1; HUMPPARG; NR1C3; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; PPAR gamma; PPARG; PPARG1; PPARG2; PPARG3; PPARG_HUMAN.
JournalPMIDIFApplicationScientific Reports (2016)271426915.5800IHC-PScientific Reports (2015)258278225.0780WBPLoS One (2014)248102493.7300WBIn Vitro Cellular & Developmental Biology-Animal (2014)249618351.2890WB
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
Phospho-PPAR Gamma (ser273)抗体交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量57kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser273:251-350/505
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-PPAR Gamma (ser273)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
Function:
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
Subunit:
Forms a heterodimer with the retinoic acid receptor RXRA called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with FAM120B. Interacts with PRDM16 (By similarity). Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 LXXLL motifs. Interacts with DNTTIP2, MAP2K1/MEK1, PRMT2 and TGFB1I1. Interacts with PDPK1. Interacts with ASXL1 AND ASXL2.
Subcellular Location:
Nucleus. Cytoplasm.
Tissue Specificity:
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
DISEASE:
Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
SWISS:
P37231
Gene ID:
5468
Phospho-PPAR Gamma (ser273)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-PPAR Gamma (ser273)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 CSGLCAT 硫酸软骨素聚合酶3抗体
合格 Transition Protein 1 真核肽链释放因子3a/eRF3抗体
合格 NMDAR1 离子型谷氨酸受体1抗体
合格 Magphinin 卵母细胞TRO抗体
合格 KCTD16 钾离子通道多聚体结构域蛋白16抗体
合格 合格 QPRT/QAPRTase 喹啉酸磷酸核糖基转移酶抗体
合格 合格 CXCR4 细胞表面趋化因子受体4抗体
合格 CXCR4 细胞表面趋化因子受体4抗体
合格 CD31 血小板内皮细胞黏附分子-1抗体
合格 CD31 血小板内皮细胞黏附分子-1抗体
合格 合格 CD31 血小板内皮细胞黏附分子-1抗体
合格 CD43 CD43抗体
合格 KCNT1 钾离子通道亚家族T成员1抗体
合格 TBCE 微管蛋白特定伴侣蛋白E抗体
合格 Anti-CCDC60 卷曲螺旋结构域蛋白60抗体
合格 FAM98B FAM98B蛋白抗体
合格 TTI1 转录因子相互作用蛋白1抗体
合格 IQSEC3 IQSEC3蛋白抗体
合格 Myelin-oligodendrocyte glycoprotein 髓鞘少树突胶质细胞糖蛋白抗体
合格 Anti-phospho-Smad2 (Ser250) 磷酸化细胞信号转导分子SMAD2抗体
合格 SPTBN2 非红细胞血影脑蛋白β2/spectrin β III抗体
合格 Calsequestrin 肌钙集蛋白/收钙素抗体
合格 合格 Lamin B 核纤层蛋白B抗体(细胞核膜标志物)
合格 Bcl-2 Bcl-2抗体