phospho-PTPN6(Ser591)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5578R
英文名称phospho-PTPN6(Ser591)
中文名称磷酸化蛋白酪氨酸磷酸酶1C抗体
别 名PTPN6(phospho S591); SHP1(phospho S591); p-SHP1(Ser591); SHP-1(Phospho-Tyr591); PTPN6(phospho Y591); PTPN6(phospho Y591); SHP1; 70 kda SHP 1L protein; 70 kda SHP1L protein; 70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP 1C; HPTP1C; MGC124580; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP 1; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; PTPN 6; PTPN6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1L; SHPTP 1; SHPTP1; Tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 发育生物学 信号转导 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-PTPN6(Ser591)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Horse,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量65kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human PTPN6 around the phosphorylation site of Ser591 [KG(p-S)LK]:KG(p-S)LK
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-PTPN6(Ser591)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
Function:
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Subunit:
Monomer. Interacts with MTUS1. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT. Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1.
Subcellular Location:
Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II. Shuttles between the cytoplasm and nucleus via its association with PDPK1.
Tissue Specificity:
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Post-translational modifications:
Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 enhances phosphatase activity.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
SWISS:
P29350
Gene ID:
5777
phospho-PTPN6(Ser591)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-PTPN6(Ser591)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 SGSM2 小G蛋白信号调节蛋白2抗体
合格 SH2D5 含SH2结构域蛋白D5抗体
合格 SH3BP1 SH3结构域结合蛋白3抗体
合格 合格 SH3BP2 SH3结构域结合蛋白2抗体
合格 phospho-SH3BP2 (Ser427) 磷酸化SH3结构域结合蛋白2抗体
合格 SH3BP5 SH3结构域结合蛋白5抗体
合格 SH3GL2/Endophilin A1 胞吞蛋白Endophilin 1抗体
合格 RBCK1/HBV associated factor 4 乙型肝炎X病毒相关蛋白4抗体
合格 RBM14 RNA结合蛋白14抗体
合格 RBM16 RNA结合蛋白16抗体
合格 RBM17 RNA结合蛋白17抗体
合格 合格 RBM22 RNA结合蛋白22抗体
合格 RBM25 RNA结合蛋白25抗体
合格 Matrilin 1 软骨基质蛋白抗体
合格 RBM34 RNA结合蛋白34抗体
合格 RBM35A/ESRP1 RNA结合蛋白35A抗体
合格 合格 RBM47 RNA结合蛋白47抗体
合格 RBMS1/Cervical cancer oncogene 4 宫颈癌癌基因4抗体
合格 RBPSUHL 转录因子RBPL抗体
合格 phospho-NFAT2 (Ser233) (Ser235 mouse) 磷酸化活化T细胞核因子1蛋白抗体
合格 phospho-NFkB p100/p52 (Ser870) 磷酸化细胞核因子p100基因结合核因子抗体
合格 Histone binding protein/SLBP 组蛋白结合蛋白SLBP抗体
合格 SLC10A6 溶质载体家族蛋白10成员A6抗体
合格 SLC1A4 溶质载体家族蛋白1成员A4抗体
合格 TMEM180 跨膜蛋白180抗体
合格 SLC22A2 溶质载体家族蛋白22成员A2抗体
合格 SLC22A7 溶质载体家族蛋白22成员A7抗体
合格 合格 SLC34A1 溶质载体家族蛋白34成员A1抗体