Phospho-RPS6 (Ser235+Ser236)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3388R
英文名称Phospho-RPS6 (Ser235+Ser236)
中文名称磷酸化S6核糖体蛋白抗体
别 名RPS6 (phospho S235 + S236); p-RPS6 (phospho S235 + S236); Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal (1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; RPS 6; RpS6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 **学 染色质和核信号 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
Phospho-RPS6 (Ser235+Ser236)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量27kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human S6 Ribosomal Protein around the phosphorylation site of Ser235/236:RL(p-S)(p-S)LR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-RPS6 (Ser235+Ser236)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Function:
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Post-translational modifications:
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphrylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Similarity:
Belongs to the ribosomal protein S6e family.
SWISS:
P62753
Gene ID:
6194
Phospho-RPS6 (Ser235+Ser236)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-RPS6 (Ser235+Ser236)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 LIMCH1 LIMCH1蛋白抗体
合格 LIMD2 LIMD2蛋白抗体
合格 IL-11 白介素11抗体
合格 LIN9 LIN9蛋白抗体
合格 LINGO2 富含亮氨酸重复跨膜神经元2抗体
合格 LINS LINS蛋白抗体
合格 LIPC 肝脂酶抗体
合格 LIPM/Lipase M 脂肪水解酶LIPM抗体
合格 DLDD/Lipoamide Dehydrogenase 硫辛酰胺脱氢酶抗体
合格 LIPT2 硫辛酰连接酶2抗体
合格 Liprin alpha 2 酪氨酸磷酸酶受体相互作用蛋白Liprin α2抗体
合格 LIX1L Lix1样蛋白抗体
合格 合格 LMAN1L 凝集素甘露糖结合蛋白1样蛋白抗体
合格 LMBRD1 核输出信号相互作用蛋白抗体
合格 IL-16 白介素16抗体
合格 LMO7 LMO7蛋白抗体
合格 LMOD1 甲状腺相关眼病自身抗原抗体
合格 LMOD2 平滑肌蛋白LMOD2抗体
合格 LMTK3 丝氨酸/苏氨酸蛋白激酶LMTK3抗体
合格 C20orf201 20号染色体开放阅读框201抗体
合格 PPARGC1A 过氧化物酶体增殖物激活受体γ辅激活子1α抗体
合格 RNF127/LONRF3 环指蛋白127抗体
合格 Vesicular Acetylcholine Transporter 囊泡乙酰胆碱通道抗体
合格 LPCAT1 溶血卵磷脂酰基转移酶1抗体