phospho-Stathmin 1 (Ser63)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 9764R
英文名称phospho-Stathmin 1 (Ser63)
中文名称磷酸化原癌基因蛋白18抗体
别 名Stathmin 1 (phospho S63); p-Stathmin 1 (phospho S63); Stathmin 1 (Phospho-Ser63); Stathmin (Phospho Ser63); Stathmin (Phospho S63); Lag; LAP 18; LAP18; Leukemia associated phosphoprotein p18; Metablastin; Oncoprotein 18; OP 18; OP18; p18; p19; Phosphoprotein 19; Phosphoprotein p19; PP17; PP19; PR22; Pr22 protein; Prosolin; Protein Pr22; SMN; Stathmin; Stathmin1; STMN 1; STMN1; STMN1_HUMAN.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞粘附分子 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
phospho-Stathmin 1 (Ser63)抗体交叉反应 Human, Mouse, Rat, Chicken, Cow, Rabbit,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量16kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human Stathmin 1 around the phosphorylation site of Ser63:41-140/149
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Stathmin 1 (Ser63)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
Function:
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Subunit:
Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST.
Subcellular Location:
Cytoplasm, cytoskeleton.
Tissue Specificity:
Ubiquitous. Expression is strongest in fetal and brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Post-translational modifications:
Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization. Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Similarity:
Belongs to the stathmin family.
Gene ID:
3925
phospho-Stathmin 1 (Ser63)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Stathmin 1 (Ser63)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
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合格 DPM3 DPM3蛋白抗体
合格 DPRX DPRX蛋白抗体
合格 DSU 视杆细胞相关蛋白MREG抗体
合格 DTHD1 死亡结构域蛋白1抗体
合格 Gastrokine 1 胃窦粘膜蛋白GKN1抗体
合格 DUS2L DUS2L蛋白抗体
合格 TLR3 Toll样受体3抗体
合格 DUSP26 双特异性磷酸酶抗体26抗体
合格 Motilin 胃动素抗体
合格 DUSP9 双特异性磷酸酶抗体9抗体
合格 DUX4L9 双同源蛋白4样蛋白9抗体
合格 DYNLT3 动力蛋白轻链Tctex-3抗体
合格 合格 HIF 2 alpha 缺氧诱导因子2α /HIF-2α/HIF-2-alpha抗体
合格 ECHDC1 ECHDC1蛋白抗体
合格 ECSIT ECSIT蛋白抗体
合格 Jagged1 CD339抗体
合格 GPR183 G蛋白偶联受体183抗体
合格 Prohibitin 抑制素/肿瘤抑制因子抗体
合格 EEFSEC 硒特异延伸因子/SELB抗体
合格 EEPD1 EEPD1蛋白抗体
合格 EFCAB1 EFCAB1蛋白抗体
合格 EFCAB3 EFCAB3蛋白抗体
合格 EFCAB4B EFCAB4B蛋白抗体
合格 EFCAB5 EFCAB5蛋白抗体
合格 EFCAB6 EFCAB6蛋白抗体
合格 EFR3B EFR3B蛋白抗体
合格 合格 EHBP1 EHBP1蛋白抗体