phospho-TNIK (Ser764)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 5598R
英文名称phospho-TNIK (Ser764)
中文名称磷酸化TRAF2和NCK激酶相互作用蛋白抗体
别 名TNIK (phospho S764); p-TNIK (phospho S764); TNIK (phospho-Ser764); TNIK (phospho-S764); p-TNIK (Ser764); p-TNIK (S764); Traf2 and NCK interacting kinase; 1500031A17RIK; 4831440I19RIK; AI451411; C530008O15Rik; C630040K21RIK; KIAA0551; MGC189819; MGC189859; RGD1561817; TNIK; TNIK_HUMAN; TRAF2 and NCK-interacting protein kinase.
说 明 书100ul
产品类型磷酸化抗体
研究领域**学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
phospho-TNIK (Ser764)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量150kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human TNIK around the phosphorylation site of Ser764:AN(p-S)KS
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-TNIK (Ser764)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
TNIK is a MSN protein kinase that interacts with both TNF receptor-associated factor 2 (TRAF2) and the adapter protein NCK. The protein has been shown to activate the c-Jun N-terminal kinase pathway when over expressed in Phoenix-A cells. TNIK has been shown to phosphorylate gelsolin, the principal intracellular and extracellular actin-severing protein, in vitro. This and evidence from mutational studies suggest that TNIK functions in the regulation of the cytoskeleton. Northern analysis indicates TNIK expression in human heart, skeletal muscle, and brain, with lower levels of expression in kidney, liver, lung, and pancreas. ESTs have been isolated from human tissue libraries, including normal amnion, gallbladder and skin.
Function:
Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
Subunit:
Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1.
Subcellular Location:
Nucleus. Cytoplasm. Recycling endosome. Cytoplasm, cytoskeleton. Note=Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
Tissue Specificity:
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
Post-translational modifications:
Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
SWISS:
Q9UKE5
Gene ID:
23043
phospho-TNIK (Ser764)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-TNIK (Ser764)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 XIAP/BIRC4 X-连锁凋亡蛋白/性连锁凋亡抑制蛋白抗体
合格 合格 STCH/HSPA13 热休克蛋白70家族13抗体
合格 STEAP3 前列腺跨膜上皮3抗原抗体
合格 STK11IP 丝氨酸/苏氨酸激酶作用蛋白11抗体
合格 STK16 丝氨酸/苏氨酸蛋白激酶16抗体
合格 ASPP1 p53凋亡刺激蛋白1抗体
合格 SURF4 过量位点蛋白4抗体
合格 合格 Bak Bcl-2同源拮抗剂抗体
合格 SYBL1 突触小泡蛋白样蛋白1抗体
合格 phospho-beta Catenin (Ser37) 磷酸化β-连环蛋白/β-连环素/β链接素抗体
合格 合格 phospho-beta Catenin (Thr41) 磷酸化β-连环蛋白/β-连环素/β链接素抗体
合格 phospho-beta Catenin (Tyr333) 磷酸化β-连环蛋白/β-连环素/β链接素抗体
合格 phospho-beta Catenin (Tyr489) 磷酸化β-连环蛋白/β-连环素/β链接素抗体
合格 ETS1 转录因子家族ETS1抗体
合格 Beta crystallin S γS-crystallin蛋白抗体
合格 beta IV Tubulin 微管蛋白β4抗体
合格 合格 TUBB5 微管蛋白β5抗体
合格 phospho-Bid (Ser78) 磷酸化BH3结构域凋亡诱导蛋白抗体
合格 合格 NR1H4 胆汁酸受体抗体
合格 合格 Biliverdin Reductase 胆绿素还原酶抗体
合格 CD40L CD40L抗体
合格 phospho-BNIP3 (Ser95) 磷酸化促凋亡调节蛋白BNIP3抗体