phospho-TOP2A (Ser1106)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3451R
英文名称phospho-TOP2A (Ser1106)
中文名称磷酸化DNA拓普西异构酶Ⅱ抗体
别 名TOP2A (phospho Ser1106); TOP2A (phospho S1106); Topoisomerase II alpha (phospho S1106); DNA topoisomeraseⅡalpha; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; TOP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TOP2A_HUMAN; Topo IIα; Topo-IIα.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
phospho-TOP2A (Ser1106)抗体交叉反应 Human, Dog, Pig, Sheep, Danio
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量174kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human TOPO II Alpha around the phosphorylation site of Ser1106:EE(p-S)DN
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-TOP2A (Ser1106)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Function:
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.
Subunit:
Homodimer. Interacts with COPS5.
Subcellular Location:
Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.
Post-translational modifications:
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
Similarity:
Belongs to the type II topoisomerase family.
Contains 1 Toprim domain.
SWISS:
P11388
Gene ID:
7153
phospho-TOP2A (Ser1106)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-TOP2A (Ser1106)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 SMYD5 SMYD5蛋白抗体
合格 SNPH 伸展蛋白SNPH抗体
合格 SNRK SNF相关丝氨酸/苏氨酸蛋白激酶抗体
合格 SNRNP200 SNRNP200蛋白抗体
合格 TMEM85 跨膜蛋白85抗体
合格 TMPRSS11D 跨膜丝氨酸蛋白酶11D抗体
合格 TMX3 硫氧还蛋白相关跨膜蛋白3抗体
合格 VAC14 VAC14蛋白抗体
合格 Macrophage Inflammatory Protein 3 alpha 巨噬细胞炎性蛋白MIP-3a抗体
合格 PKNOX1 同源盒蛋白PKNOX1抗体
合格 合格 Doublecortin 双皮质素抗体
合格 phospho-DRP1 (Ser616) 磷酸化动力相关蛋白1抗体
合格 B4GALNT1/GM2 synthase β1,4-N乙酰半乳糖氨基转移酶1抗体
合格 CK16 细胞角蛋白16抗体
合格 合格 合格 phospho-IRS1 (Ser616) 磷酸化胰岛素受体底物1抗体
合格 PLD3 胆碱磷酸酯酶3抗体
合格 HTRA2 线粒体丝氨酸蛋白酶抗体
合格 PLEKHA1 含普列克底物蛋白家族A成员1抗体
合格 合格 E-Selectin E选择素抗体
合格 PMM2 磷酸甘露糖变位酶2抗体
合格 PNMA5 类肿瘤性抗原MA5抗体
合格 PNPLA3 脂肪营养素PNPLA3抗体
合格 Integrin alpha E 整合素αE抗体
合格 PODNL1 PODN样蛋白1抗体