phospho-Transferrin Receptor (Ser24)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 16668R
英文名称phospho-Transferrin Receptor (Ser24)
中文名称磷酸化转铁蛋白受体抗体
别 名Transferrin Receptor (phospho S24); p-Transferrin Receptor (phospho S24); CD 71; CD71; CD71 antigen; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; p90; sTfR; T9; TFR 1; TFR; TFR1; TFR1_HUMAN; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; Transferrin receptor protein 1, serum form; TRFR.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 信号转导 干细胞 转录调节因子 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
phospho-Transferrin Receptor (Ser24)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量85kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human Transferrin Receptor around the phosphorylation site of Ser24:RF(p-S)LA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Transferrin Receptor (Ser24)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.
Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Subcellular Location:
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications:
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Similarity:
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
SWISS:
P02786
Gene ID:
7073
phospho-Transferrin Receptor (Ser24)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Transferrin Receptor (Ser24)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 PDE9A 磷酸二酯酶9A抗体
合格 ICAM-2/CD102 细胞间粘附分子-2抗体
合格 CRTR1 CP2相关转录抑制因子1抗体
合格 phospho-PDCD4 (Ser67) 磷酸化凋亡相关蛋白4抗体
合格 PDIA6 蛋白二硫异构酶A6抗体
合格 PDLIM2 PDLIM2蛋白抗体
合格 PECI PECI蛋白抗体
合格 合格 PELI3 PELI3蛋白抗体
合格 PEPD 脯氨酸二肽酶抗体
合格 PEPP2 磷酸肌醇3磷酸结合蛋白2抗体
合格 PEX11B 过氧化物酶体生物合成因子11B抗体
合格 PEX5 过氧化物酶体生物合成因子5抗体
合格 PEX6 过氧化物酶体生物合成因子6抗体
合格 PFDN3 希佩尔-林道结合蛋白抗体
合格 DAP12 自然杀伤激活受体相关蛋白DAP12抗体
合格 PFKFB3/PFK2 果糖-2,6-二磷酸酶3/磷酸果糖激酶2抗体
合格 SMCR7 SMCR7蛋白抗体
合格 SMG5 SMG5蛋白抗体
合格 SMPDL3B 酸性鞘磷脂酶样磷酸二酯酶3B抗体
合格 SMUG1 SMUG1蛋白抗体
合格 SMYD1 SMYD1蛋白抗体