phospho-TRF1 (Ser219)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy-
16714R
英文名称phospho-TRF1 (Ser219)
中文名称磷酸化端粒体复制结合因子1抗体
别 名TRF1 (phospho S219); p-TRF1 (phospho S219); hTRF1 AS; NIMA interacting protein 2; NIMA-interacting protein 2; PIN 2; PIN2; t TRF1; Telomeric protein Pin2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor (NIMA interacting) 1; Telomeric repeat binding factor 1; Telomeric repeat binding protein 1; Telomeric repeat-binding factor 1; TERF 1; TERF1; TERF1_HUMAN; TRBF 1; TRBF1; TRF 1; TRF; TTAGGG repeat binding factor 1; TTAGGG repeat-binding factor 1.
说 明 书100ul 200ul
研究领域
抗体来源Rabbit
克隆类型Polyclonal
phospho-TRF1 (Ser219)抗体交叉反应 Human, Horse, Rabbit, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量50kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human TRF1 around the phosphorylation site of Ser219:161-260/439
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-TRF1 (Ser219)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
Function:
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Subcellular Location:
Nucleus. Cytoplasm; cytoskeleton; spindle. Chromosome; telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
Tissue Specificity:
Highly expressed and ubiquitous. Isoform Pin2 predominates.
Post-translational modifications:
Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Similarity:
Contains 1 HTH myb-type DNA-binding domain.
SWISS:
P54274
Gene ID:
7013
phospho-TRF1 (Ser219)抗体（antibody, Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-TRF1 (Ser219)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Apolipoprotein O 载脂蛋白O抗体
合格 APPBP1 β淀粉样蛋白前体蛋白结合蛋白1抗体
合格 ARFRP1 ADP核糖基化因子相关蛋白1
合格 Argininosuccinate Lyase 琥珀酸裂解酶抗体
合格 Argonaute 4 真核翻译起始因子2C4抗体
合格 合格 CD166 活化白细胞粘附分子抗体
合格 合格 ARIH2 E3连接酶蛋白ARIH2抗体
合格 ARL4 ADP核糖基化因子样蛋白4抗体
合格 ARP2 肌动蛋白相关蛋白2/3抗体
合格 合格 ARPC1A 肌动蛋白相关蛋白2/3亚型1A抗体
合格 phospho-ATF2 (Thr51 + Thr53) 磷酸化活化复制因子2抗体
合格 AQP3 水通道蛋白-3抗体
合格 phospho-ATM (Ser794) 磷酸化****扩张性共济失调症突变蛋白抗体
合格 ATP6V0D2 ATP6V0D2蛋白抗体
合格 ATPIF1 ATPIF1蛋白抗体
合格 Phospho-Aurora B (Tyr12) 磷酸化有丝分裂激酶B抗体
合格 phospho-B Raf (Thr598 + Ser601) 磷酸化B-Raf抗体
合格 合格 Phospho-Bcl2 (Ser87) 磷酸化Bcl-2抗体
合格 合格 Phospho-Bcl2 (Thr69) 磷酸化Bcl-2抗体
合格 phospho-Bcl-xL (Thr47) 磷酸化Bcl-xL蛋白抗体
合格 合格 FGF5 纤维母细胞生长因子5抗体
合格 合格 phospho-beta Catenin (Ser33) 磷酸化β-连环蛋白/β-连环素/β链接素抗体