Phospho-Vimentin (Ser56)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3471R
英文名称Phospho-Vimentin (Ser56)
中文名称磷酸化波形蛋白抗体
别 名Vimentin (phospho S56); p-Vimentin (phospho S56); FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.
说 明 书100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 发育生物学 神经生物学 信号转导 干细胞 细胞骨架 肿瘤细胞生物标志物
抗体来源Rabbit
克隆类型Polyclonal
Phospho-Vimentin (Ser56)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量51kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56:S(p-S)PG
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Vimentin (Ser56)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Function:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Subunit:
Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain).Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Highly expressed in fibroblasts, somev expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Post-translational modifications:
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P08670
Gene ID:
7431
Phospho-Vimentin (Ser56)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Vimentin (Ser56)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 HSPABP/HIP/HSC70 Interacting Protein HIP 热休克蛋白70相互作用蛋白抗体
合格 WFS1 Wolfram综合征蛋白1抗体
合格 Complement C4 / C4a 过敏**C4/补体C4抗体
合格 合格 Dmrta1 性发育转录因子蛋白DMO抗体
合格 DNAJA1 热休克蛋白相关蛋白4抗体
合格 合格 phospho-E2F1 (Thr433) 磷酸化转录因子E2F1抗体
合格 GBP2 G蛋白结合蛋白2抗体
合格 合格 GBP2 G蛋白结合蛋白2抗体
合格 PHC2 早期发育调控蛋白2/HDC2抗体
合格 NAB2 EGR1结合蛋白2抗体
合格 PTPN6 造血细胞磷酸酶抗体
合格 INSL3 胰岛素样蛋白3抗体
合格 RFC2 复制激活因子C2抗体
合格 Prostaglandin I2 Receptor 前列环素受体抗体
合格 合格 TPOR 血小板生成素受体抗体
合格 CLIP2 细胞质连接蛋白2抗体
合格 SNAP25 突触相关蛋白25抗体
合格 合格 Neuroglycan C 跨膜硫酸软骨素蛋白聚糖C抗体
合格 Nova2 神经肿瘤Nova2抗原抗体
合格 STEP 神经特异蛋白酪氨酸磷酸酶N5抗体
合格 CNGB1 环核苷酸门控阳离子通道蛋白CNG-1β抗体
合格 AP180 装配衔接蛋白180抗体
合格 APBA1 β淀粉样前体蛋白结合蛋白1抗体（X11α）
合格 CASK 钙/钙调蛋白依赖性丝氨酸蛋白激酶抗体
合格 SPARC 富含半胱氨酸的酸性分泌蛋白抗体
合格 CPEB3 细胞质多聚腺苷酸结合蛋白3抗体