PIBF1抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。
产品编号xy- 9612R
英文名称PIBF1
中文名称孕酮诱导阻断因子1抗体
别 名PIBF 1; PIBF; PIBF1; PIBF-1; Progesterone immunomodulatory binding factor 1; Progesterone induced blocking factor 1; Progesterone-Induced Blocking Factor 1; RP11 505F3.1; PIBF1_HUMAN; C13orf24.
说 明 书100ul 200ul
研究领域细胞生物 **学 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
PIBF1抗体交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量90kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PIBF1/C13orf24:551-651/757
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
PIBF1抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
PIBF is synthesized during pregnancy in response to progesterone by progesterone receptor-positive T lymphocytes (mostly gamma-delta T cells). In the presence of PIBF, natural killer (NK) cells inhibit the release of perforin from storage granules and therefore fail to lyse target cells. In humans, the amount of cells that express PIBF is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. Full-length PIBF is associated with the nucleus, whereas secretion of shorter forms is induced by activation of the cell. Research suggests that PIBF functions as a transcription factor in its full-length form, while smaller forms may act as cytokines. The PIBF gene encodes a deduced hydrophilic 757-amino acid alpha-helical protein with an N-terminal signal sequence, a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, an endoplasmic reticulum membrane retention signal, and many presumeed N-glycosylation and phosphorylation sites.
Function:
Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.
Tissue Specificity:
Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon.
Gene ID:
10464
PIBF1抗体(antibody,
Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。
PIBF1抗体普通抗体重链和轻链的结构
重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构: 普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1,IgG2,IgG3,IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为2:1,而在小鼠的比例为20:1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen binding,Fab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段(fragment crystallizable,Fc)相当于Ig的CH2和CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域,相当于Y字结构下面那一部分。
合格 MEK1 + MEK2 丝裂原活化蛋白激酶激酶1抗体
合格 合格 Collagen I I型胶原蛋白/胶原蛋白1/1型胶原蛋白/I型胶原a1抗体
合格 C2a 补体C2a链多肽抗体
合格 beta Amyloid 1-28 β淀粉样肽1-28/β-Amyloid 1-28抗体
合格 CD90 CD90抗体
合格 CACNB3 L型电压依赖型钙通道β3(L-type Ca++ CPβ3)抗体
合格 NR0B1 肾上腺发育不全相关蛋白抗体
合格 Tex14 癌/睾丸抗原113抗体
合格 B3GALNT1 β1,3半乳糖转移酶3抗体
合格 phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) 磷酸化丝氨酸/苏氨酸蛋白激酶MST4,MST3,STK25抗体
合格 合格 Caspase-1 p20 天冬氨酸-胱氨酸特异性蛋白酶家族抗体
合格 STOM 红细胞膜蛋白STOM抗体
合格 NSE 神经元特异性烯醇化酶/γ 烯醇化酶抗体
合格 HPV16 E7 人类**状瘤病毒16-E7抗体
合格 合格 phospho-HSP70 (Tyr611) 磷酸化热休克蛋白-70抗体
合格 phospho-HSP70 (Tyr41) 磷酸化热休克蛋白-70抗体
合格 Phospho-CD18 (Ser756) 磷酸化整合素β2/Integrin β2抗体
合格 合格 Phospho-CD18 (Thr758) 磷酸化整合素β2/Integrin β2抗体
合格 合格 ADRA1B alpha 1肾上腺素能受体B抗体
合格 DRAK2 DAP凋亡诱导蛋白激酶2抗体
合格 CCL4 巨噬细胞炎症因子1β /MIP-1β抗体
合格 DYNC1I1 胞浆动力蛋白中间链1抗体
合格 DYNC1I2 胞浆动力蛋白中间链2抗体
合格 合格 CATSPER2 阳离子通道精子相关蛋白2抗体
合格 合格 Serine racemase 丝氨酸消旋酶抗体