Piwil2抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 3817R
英文名称Piwil2
中文名称piwi样2蛋白抗体
别 名HILI; Hiwi like; MILI; Miwi like; Piwi like 2; Piwi-like 2; Piwil 2; Piwil-2; Piwil2; Cancer/testis antigen 80; CT80; PIWL2_HUMAN.
JournalPMIDIFApplicationCancer Immunology, Immunotherapy (2013)240778523.6370WB
说 明 书100ul 200ul
研究领域肿瘤 细胞生物 **学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
Piwil2抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Guinea Pig,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量107kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Piwil2/Mili:885-973/973
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Piwil2抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
HILI is a 973 amino acid protein encoded by the human gene PIWIL2. HILI belongs to the argonaute family and contains one PAZ domain and one PIWI domain. HILI is a cytoplasmic protein that is expressed in ***** testis and in most tumors. It regulates spermatogenesis and primordial germ cell production and has an essential role in meiotic differentiation of spermatocytes and in self-renewal of spermatogonial stem cells. Expression of HILI can modulate expression of genes involved in stem cell proliferation (such as PDGFR-b), in energy metabolism (such as Glut1), in cell-cell interaction (such as Integrin a6, GJA7, THY-1 and CD9), and in germ cell differentiation (such as STRA8). It may also play a role as a regulatory factor of Stat3/Bcl-xS/L/CCND1 pathway. Repression of HILI can inhibit tumor cell growth. HILI acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
Function:
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
Subunit:
Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1.
Subcellular Location:
Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
Tissue Specificity:
Expressed in ***** testis and in most tumors.
Post-translational modifications:
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
Similarity:
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
SWISS:
Q8TC59
Gene ID:
55124
Piwil2抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Piwil2抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 Cardiac Troponin I/TNNC1 心肌肌钙蛋白抗体
合格 BRCA1 乳腺癌易感基因1抗体
合格 BRCA1 乳腺癌易感基因1抗体
合格 CD56 神经细胞粘附分子1抗体
合格 Collagen IV IV型胶原蛋白/4型胶原蛋白/胶原蛋白4抗体
合格 Fibulin 5 衰老关键蛋白抗体
合格 IL-1 Beta 白介素1β/IL-1β抗体
合格 Casein 酪蛋白抗体
合格 Megsin 丝氨酸蛋白酶抑制剂B7抗体
合格 Lysozyme 溶菌酶抗体
合格 MAGE-1 黑色素瘤相关抗原抗体
合格 Tyrosinase 酪氨酸酶抗体
合格 laminin 层粘连蛋白抗体
合格 合格 TTF1 甲状腺核转录因子-1抗体
合格 Thrombin receptor/ PAR1 蛋白酶激活受体-1抗体
合格 Nanog 胚胎干细胞关键蛋白抗体
合格 MHC I类链相关蛋白A/组织相容性复合物MHCIa抗体
合格 Glutathione Reductase 谷胱甘肽还原酶抗体
合格 Myc tag c-Myc tag标签抗体
合格 APOA1 载脂蛋白A1抗体
合格 Insulin 胰岛素抗体
合格 GRO Alpha 黑色素瘤生长刺激活性蛋白α抗体 GROa
合格 合格 beta Amyloid 31-35 β淀粉样肽（31-35)/Aβ 31-35 抗体
合格 beta-Amyloid(25-35) β淀粉样肽（25-35）抗体