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PKC delta抗体

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产品名称: PKC delta抗体
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产品展商: XYbscience
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简单介绍

PKC delta抗体是一个家庭的丝氨酸-苏氨酸蛋白激酶,可通过钙第二信使甘油二酯激活。PKC家族成员磷酸化多种靶蛋白是已知的参与不同的细胞信号通路。PKC家族成员也是佛波醇酯的主要受体。PKC家族的每个成员都有特定的表达谱,被认为在细胞中扮演着不同的角色。该基因编码的蛋白质是PKC家族成员之一。PKC delta抗体人体和小鼠的研究表明,这种激酶参与B细胞信号转导,并参与多种细胞类型的生长、凋亡和分化的调节。交替剪接转录变体已观察到相同的蛋白质。[由RefSeq提供,月2008日]。


PKC delta抗体  的详细介绍

PKC delta抗体特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而,抗体可通过与病毒或**的特异性结合,直接发挥中和病毒的作用。

产品编号xy-23921R

英文名称PKC delta

中文名称蛋白激酶C亚性D型抗体

别    名MAY 1; MAY1; nPKC delta; PCKd; PKC d; PKC delta; PKC-d; PKCD; PKCdelta; PRKC D; PRKC delta; PRKC-d; PRKCd; Protein Kinase C delta; Protein kinase C delta type; Protein Kinase Cdelta; KPCD_HUMAN.  

说 明 书50ul  100ul  200ul

研究领域肿瘤  细胞生物  细胞凋亡  转录调节因子  激酶和磷酸酶  

抗体来源Rabbit

克隆类型Polyclonal

PKC delta抗体交叉反应 Human, Mouse, Rat,

产品应用WB=1:500-1000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量77kDa

细胞定位细胞核 细胞浆 细胞膜

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human PKC delta:

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

PKC delta抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

产品介绍background:

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008].


Function:

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Is highly expressed in a number of cancer cells and promotes cell survival and resistance against chemotherapeutic drugs by inducing cyclin D1 (CCND1) and hyperphosphorylation of RB1, and via several pro-survival pathways, including NF-kappa-B, AKT1 and MAPK1/3 (ERK1/2). Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.


Subunit:

Interacts with PDK1 (via N-terminus region), RAD9A, CDCP1, MUC1 and VASP.


Subcellular Location:

Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Endoplasmic reticulum. Mitochondrion. Cell membrane; Peripheral membrane protein.


Post-translational modifications:

Autophosphorylated and/or phosphorylated at Thr-507, within the activation loop; phosphorylation at Thr-507 is not a prerequisite for enzymatic activity. Autophosphorylated at Ser-299, Ser-302 and Ser-304. Upon TNFSF10/TRAIL treatment, phosphorylated at Tyr-155; phosphorylation is required for its translocation to the endoplasmic reticulum and cleavage by caspase-3. Phosphorylated at Tyr-313, Tyr-334 and Tyr-567; phosphorylation of Tyr-313 and Tyr-567 following thrombin stimulation potentiates its kinase activity. Phosphorylated by protein kinase PDK1; phosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2.


Similarity:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.


SWISS:

Q05655


Gene ID:

5580

PKC delta抗体antibody, Ab)是由效应B细胞(效应**B细胞)分泌,机体用于抵御外来物质,如病毒,**等抗原,结构呈“Y”字型的球状蛋白质,仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质,均被称作抗原,因此对于抗抗体(能够结合抗体的抗体)来说,抗体本身也是一种抗原物质。

   QQ图片20171030091318

PKC delta抗体普通抗体重链和轻链的结构

重链结构:普通的**球蛋白具有2条重链(H链),分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型,对应的**球蛋白名称分别为IgMIgGIgAIgDIgE

轻链结构:  普通**球蛋白具有2条轻链(L链),分子质量约25kDa,有κ链和λ链两种亚型,这两种轻链决定了Ig的亚型类别(IgG1IgG2IgG3IgG4)。一个天然的Ig分子两条轻链总是相同的,但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同,正常人血清**球蛋白κ链:λ链约为21,而在小鼠的比例为201

2.2抗体Fab段和Fc

IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1Fc,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应;经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段(fragment of antigen bindingFab),相当于抗体分子的两个臂,由一个完整的轻链和重链的VHCH1结构域组成。Fc段为可结晶段(fragment crystallizableFc)相当于IgCH2CH3结构域,是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区,以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2CH3的区域,相当于Y字结构下面那一部分。

合格 B7H4 B7-H4抗体
合格 FGFR2 成纤维细胞生长因子受体2抗体
合格 合格 Angiopoietin 2 血管生成素2抗体
合格 SYVN1 滑膜细胞凋亡抑制物1抗体
合格 IGF1R 胰岛素样生长因子1受体抗体
合格 Insulin Receptor 胰岛素受体抗体
合格 合格 LRRK2 富亮氨酸重复激酶2抗体
合格 合格 CD147 细胞外基质金属蛋白酶诱导因子抗体
合格 合格 SYK 非受体型酪氨酸蛋白激酶抗体
合格 PTEN 一种肿瘤抑制基因抗体(C端)
合格 OAS1 寡腺苷酸合成酶-1
合格 PEPT1 肠道肽转运蛋白1/小肽转运蛋白1抗体
合格 Ceramide glucosyltransferase 谷氨酸半胱氨酸γ合成酶抗体
合格 合格 LDL receptor 低密度脂蛋白受体抗体
合格 NF-H 高分子量神经丝蛋白抗体
合格 Collagen  II Ⅱ型胶原α1蛋白/软骨钙素抗体
合格 合格 NF-M 中分子量神经丝蛋白抗体
合格 FAM3B 胰腺衍生因子抗体
合格 Beta Tubulin 微管蛋白抗体
合格 Smad2 细胞信号转导分子Smad-2抗体
合格 CEA 癌胚抗原抗体
合格 Estrogen Receptor alpha 雌**受体α抗体
合格 HSP27 热休克蛋白27/HSP25抗体
合格 合格 Cyclooxygenase 2 环氧合酶2/前列腺素内过氧化物合成酶2抗体
合格 ADAMTS7(NT) 整合素样金属蛋白酶与凝血酶1型-7抗体 (N端抗体)
 

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