PKN1抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 7478R
英文名称PKN1
中文名称蛋白激酶C相关激酶1抗体
别 名DBK; PAK 1; PAK-1; PAK1; PKC1; PKN ALPHA; PKN; Pkn1; PKN1_HUMAN; PRK1; PRKCL1; Protease activated kinase 1; Protease-activated kinase 1; Protein kinase C like 1; Protein kinase C like PKN; Protein kinase C related kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase N1; Protein kinase PKN alpha; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine threonine kinase N; Serine threonine protein kinase N; Serine-threonine protein kinase N; Serine/threonine protein kinase N1; Serine/threonine-protein kinase N1.
说 明 书100ul 200ul
研究领域细胞生物 信号转导 激酶和磷酸酶 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
PKN1抗体交叉反应 Human, Mouse, Rat, Dog, Cow,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量104kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PKN1:201-300/942
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
PKN1抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Function:
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Subcellular Location:
Cytoplasm. Nucleus. Endosome. Cell membrane. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.
Tissue Specificity:
Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
Post-translational modifications:
Autophosphorylated; preferably on serine. Phosphorylated during mitosis. Activated by limited proteolysis with trypsin.
Similarity:
Belongs to the protein kinase superfamily.
AGC Ser/Thr protein kinase family.
PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats.
Gene ID:
5585
PKN1抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
PKN1抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 CD24 CD24抗体
合格 IL-18 白细胞介素-18/干扰素γ诱导因子抗体
合格 合格 PPAR gamma 过氧化酶活化增生受体γ抗体
合格 合格 Matriptase 蛋白裂解酶（一种新的癌基因）抗体
合格 合格 TFF3 三叶肽因子3抗体
合格 Interferon alpha 2b 干扰素α2b抗体
合格 ANGPTL2 血管生成素样蛋白2抗体
合格 CAMK2A + CAMK2B + CAMK2D 钙/钙调素依赖蛋白激酶2b/2γ抗体
合格 CDK1 周期素依赖性激酶1抗体
合格 合格 SCF 干细胞生长因子抗体
合格 Endostatin 内皮抑素/内皮他丁抗体
合格 Collagen X Ⅹ型胶原抗体
合格 HMGA2 高迁移率族蛋白A2抗体
合格 Mitofusin 1 线粒体融合蛋白1抗体
合格 EMP-1 表皮膜蛋白1抗体
合格 CDK5 周期素依赖性激酶5抗体
合格 IL-13 白介素13抗体
合格 CD62p P选择素/白细胞内皮细胞粘附分子3抗体
合格 CCR-2 细胞表面趋化因子受体2抗体
合格 MDR1 多药耐药蛋白/P-糖蛋白抗体
合格 CaMK2 alpha 钙/钙调素依赖蛋白激酶2α抗体
合格 合格 合格 VEGFR2 血管内皮生长因子受体2抗体
合格 MADH7/Smad7/Smad6 Smad7抗体
合格 CDK6 周期素依赖性激酶6抗体
合格 CDK7 周期素依赖性激酶7抗体