PPARGC1A抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy-1832R
英文名称PPARGC1A
中文名称过氧化物酶体增殖物激活受体γ辅激活子1α抗体
别 名LEM6; PGC-1 Alpha; PGC1 Alpha; Ligand effect modulator 6; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1 ; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPARGC 1 alpha; PPARGC1A.
JournalPMIDIFApplicationACS Nano (2014)2481099713.0250WBToxicology (2012)226778864.0170WB
说 明 书50ul 100ul 200ul
研究领域**学 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
PPARGC1A抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量88kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PGC-1:601-700/798 23
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
PPARGC1A抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene.
Function:
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.
Subunit:
Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with PRDM16. Interacts with LRPPRC. Homooligomer. Interacts with LPIN1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
Post-translational modifications:
hosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
Similarity:
Contains 1 RRM (RNA recognition motif) domain.
SWISS:
Q9UBK2
Gene ID:
10891
PPARGC1A抗体（antibody, Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
PPARGC1A抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 GLCCI1 糖皮质**诱导转录蛋白1抗体
合格 Grpa 半乳糖凝集素相关蛋白A抗体
合格 HEXDC 氨基己糖苷酶D抗体
合格 HHLA2 HHLA2蛋白抗体
合格 合格 VAT1L 囊泡胺转运蛋白1家族蛋白抗体
合格 合格 BPIL1 **通透性增加蛋白样1抗体
合格 BPIL2 **/通透性增加蛋白样2抗体
合格 BRPF3 BRPF3蛋白抗体
合格 BTNL3 嗜乳脂蛋白样3抗体
合格 BXDC2 BXDC2蛋白抗体
合格 phospho-c-ABL1+2 (Tyr393+439) 磷酸化非受体酪氨酸激酶c-Abl抗体
合格 合格 phospho-C-Myc(Ser373) 磷酸化致癌基因C-Myc抗体
合格 FNDC1 Ⅲ型纤维连接蛋白域蛋白1抗体
合格 MX2 + MX1 干扰素诱导GTP结合蛋白MX2抗体
合格 APLP2 淀粉样蛋白β前体样蛋白2抗体
合格 合格 APLP1 淀粉样蛋白β前体样蛋白1抗体
合格 FBXL17 FBXL17蛋白抗体
合格 合格 FBXO36 FBXO36 F-box蛋白相关蛋白36抗体
合格 FBXO33 F-box蛋白相关蛋白33抗体
合格 FBXL15 FBXL15蛋白抗体
合格 MST1/MST2 蛋白激酶MST1/2抗体
合格 NMDA epsilon 2 谷氨酸受体NMDAζ2抗体
合格 FBXO18 F-box蛋白家族FBXO18抗体
合格 RNF211 环指蛋白211抗体