PRMT6抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy- 6063R
英文名称PRMT6
中文名称组蛋白精氨酸甲基转移酶6抗体
别 名PRMT 6; PRMT6; ANM6_HUMAN; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase like 6; HRMT1L6; prmt6; Protein arginine methyltransferase 6; Protein arginine N methyltransferase 6; Protein arginine N-methyltransferase 6.
说 明 书100ul 200ul
研究领域染色质和核信号 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
PRMT6抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量42kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PRMT6
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
PRMT6抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
Function:
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
Subunit:
Interacts with (and methylates) HIV-1 Tat, Rev and Nucleocapsid protein p7 (NC).
Subcellular Location:
Nucleus.
Tissue Specificity:
Highly expressed in kidney and testes.
Post-translational modifications:
Automethylated.
Similarity:
Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily.
SWISS:
Q96LA8
Gene ID:
55170
PRMT6抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
PRMT6抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 CDCP1 CD318抗体
合格 合格 Endomucin 内皮粘蛋白EMCN抗体
合格 Lumican 基膜聚糖蛋白抗体
合格 ST14 丝氨酸蛋白酶14/膜型丝氨酸蛋白酶1抗体
合格 合格 HIF3 alpha 缺氧诱导因子3α/HIF-3α抗体
合格 HIFPH4 缺氧诱导因子脯氨酰4羟化酶抗体
合格 ADORA2B 腺苷A2b受体/神经生长因子1受体抗体
合格 CYPA 亲环蛋白（亲环素）PPIA抗体
合格 合格 Calreticulin 钙网蛋白抗体
合格 Annexin A7 膜粘连蛋白7抗体
合格 ANGPTL3 血管生成素样蛋白3抗体
合格 ANP32C 致瘤磷蛋白32相关蛋白1抗体
合格 KLHL7 kelch样蛋白7抗体
合格 PBOV1 前列腺癌和乳腺癌高表达蛋白抗体
合格 SERINC3 丝氨酸合并蛋白3抗体（肿瘤差异表达蛋白）
合格 RNF56 环指蛋白56抗体
合格 RASA3 Ras-GTP酶激活蛋白3抗体
合格 Styk1 丝氨酸/苏氨酸/酪氨酸激酶STYK1抗体
合格 B MyB 转录因子MYB相关蛋白B抗体
合格 合格 NARG2 谷氨酸受体调节蛋白2抗体
合格 PLAG1 多型性腺瘤基因1蛋白抗体
合格 SIRT7 沉默调节样蛋白SirT7抗体
合格 合格 AARE 氧化蛋白质水解酶
合格 DAB2IP Dab2相互作用蛋白抗体
合格 DAB2 信号转导功能磷蛋白DAB2抗体
合格 HSP40 热休克蛋白家族40抗体