Pro-neuregulin-1, membrane-bound isoform抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产品编号xy-
1817R
英文名称Pro-neuregulin-1, membrane-bound isoform
中文名称神经胶质生长因子/神经调节蛋白β抗体
别 名Acetylcholine receptor-inducing activity; ARIA; Breast cancer cell differentiation factor p45; CHICK, homolog of; GGF; GGF2; Glial growth factor; Heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); heregulin, alpha; HGL; HRG; HRG1; HRGA; MST131; NDF; Neu differentiation factor; neuregulin 1 type IV beta 1a; neuregulin 1 type IV beta 3; NRG 1; nrg1; NRG1 beta; NRG1_HUMAN; Pro-neuregulin-1, membrane-bound isoform; Pro-NRG1; Sensory and motor neuron-derived factor; SMDF; NRG1_MOUSE.
说 明 书50ul 100ul 200ul
研究领域肿瘤 信号转导
抗体来源Rabbit
克隆类型Polyclonal
Pro-neuregulin-1, membrane-bound isoform抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Rabbit,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量71kDa
细胞定位细胞核 细胞膜 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from mouse HRG Beta1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Pro-neuregulin-1, membrane-bound isoform抗体产品介绍background:
NRG1 (Neuregulin 1) is a ligand for ERBB3 and ERBB4 tyrosine kinase receptors. NRG1 concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. There are multiple isoforms of NRG1, the type I isoforms (isoforms 1-8) have an Ig domain and a glycosylation domain. There are 10 known isoforms in total which perform distinct tissue-specific functions. Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in nonneuronal organs, whereas isoform 5 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain.
Function:
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.
Subunit:
The cytoplasmic domain interacts with the LIM domain region of LIMK1. Interacts with ERBB3 and ERBB4.
Subcellular Location:
Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Note=Does not seem to be active.
Neuregulin-1: Secreted.
Isoform 8: Nucleus. Note=May be nuclear.
Isoform 9: Secreted. Note=Has a signal peptide.
Isoform 10: Membrane; Single-pass type I membrane protein. Note=May possess an internal uncleaved signal sequence.
Tissue Specificity:
Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in ***** heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in ***** heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific.
Post-translational modifications:
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
DISEASE:
Note=A chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with TENM4. The translocation fuses the 5'-end of TENM4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines.
Similarity:
Belongs to the neuregulin family.
Contains 1 EGF-like domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
SWISS:
Q02297
Gene ID:
211323
Pro-neuregulin-1, membrane-bound isoform抗体（antibody, Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“Y”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Pro-neuregulin-1, membrane-bound isoform抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
IgG经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 phospho-P53(Thr55) 磷酸化肿瘤抑制基因P53抗体
合格 phospho-TNIK (Ser764) 磷酸化TRAF2和NCK激酶相互作用蛋白抗体
合格 phospho-TERT(Ser1125) 磷酸化端粒酶逆转录酶抗体
合格 Phospho-Tuberin(Ser1418) 磷酸化结节性硬化蛋白抗体
合格 Phospho-Smad3 (Ser425) 磷酸化细胞信号转导分子SMAD3抗体
合格 phospho-Alpha synuclein (Tyr136) 磷酸化核突触蛋白α抗体
合格 phospho-Alpha synuclein(Ser129) 磷酸化核突触蛋白α抗体
合格 phospho-SYN1(Ser62+Ser67) 磷酸化神经突触素1抗体
合格 Phospho-SHP2 (Tyr81) 磷酸化蛋白酪氨酸磷酸酶2抗体
合格 phospho-SOX9(Ser181) 磷酸化转录因子SOX9蛋白抗体
合格 phospho-c-Raf(Ser339) 磷酸化原癌基因c-Raf抗体
合格 phospho-RAD9(Tyr28) 磷酸化细胞周期检查控制蛋白质抗体
合格 phospho-NFKB p65(Thr435) 磷酸化细胞核因子抗体
合格 phospho-NFKB p65(Thr254) 磷酸化细胞核因子抗体
合格 phospho-NFKB p65(Thr505) 磷酸化细胞核因子抗体
合格 phospho-RPS6KB1(Ser417) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-RPS6KB1(Ser427) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-RPS6KB1(Ser441+Thr444) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-ROCK2(Tyr256) 磷酸化Rho相关蛋白激酶2抗体